DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 43 (DDX43) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 43 (DDX43)

Synonyms: ct13, hage, ddx53, MGC145555, ENSMUSG00000070291, OTTMUSG00000019690, zgc:174910

Clonality: Monoclonal (3G12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396075

Additional Information

Alternative name: DDX43

Gene ID: 55510

UniProt: NP_061135

Immunogen: DDX43 (NP_061135, 2 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3G12

Description: Other names: DKFZp434H2114, HAGEDEAD (Asp-Glu-Ala-Asp) box polypeptide 43

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DDX43 (NP_061135, 2 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 72844 DA

Comments:

Background: The protein encoded by this gene is an ATP-dependent RNA helicase in the DEAD-box family and displays tumor-specific expression.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Concentration: 0.5mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of DDX43 expression in transfected 293T cell line by DDX43 monoclonal antibody (M07), clone 3G12. Lane 1: DDX43 transfected lysate(72.9 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Roman-Gomez, Jimenez-Velasco, Agirre et al.: "Epigenetic regulation of human cancer/testis antigen gene, HAGE, in chronic myeloid leukemia." in: Haematologica, Vol. 92, Issue 2, pp. 153-62, 2007 (PubMed).

Mathieu, Knights, Pawelec et al.: "HAGE, a cancer/testis antigen with potential for melanoma immunotherapy: identification of several MHC class I/II HAGE-derived immunogenic peptides." in: Cancer immunology, immunotherapy : CII, Vol. 56, Issue 12, pp. 1885-95, 2007 (PubMed).

Mathieu, Linley, Reeder et al.: "HAGE, a cancer/testis antigen expressed at the protein level in a variety of cancers." in: Cancer immunity, Vol. 10, pp. 2, 2010 (PubMed).