Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1) (AA 1-272) antibody

Details for Product No. ABIN396077
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Antigen
Synonyms AAF-44, AAF44, RP11-541N10.2, RPA-32, STN1, bA541N10.2, 0610009H20Rik, 2310057J23Rik, AI413458, zgc:64174, ATSTN1, F10K1.17, F10K1_17
Epitope
AA 1-272
(9), (8), (6), (3), (2), (1), (1), (1)
Reactivity
Human
(28), (2)
Host
Mouse
(19), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(26), (16), (3), (3), (3), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN396077
450.00 $
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Immunogen OBFC1 (AAH17400, 1 a.a. ~ 272 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G12-1B7
Isotype IgG2a kappa
Specificity OBFC1 (AAH17400, 1 a.a. ~ 272 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name OBFC1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 42119 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106, MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1) (AA 1-272) antibody OBFC1 monoclonal antibody (M01), clone 3G12-1B7 Western Blot analysis of OBFC1 expression in Jurkat.
Background publications Vasan, Larson, Aragam et al.: "Genome-wide association of echocardiographic dimensions, brachial artery endothelial function and treadmill exercise responses in the Framingham Heart Study." in: BMC medical genetics, Vol. 8 Suppl 1, pp. S2, 2007 (PubMed).

Casteel, Zhuang, Zeng et al.: "A DNA polymerase-{alpha}{middle dot}primase cofactor with homology to replication protein A-32 regulates DNA replication in mammalian cells." in: The Journal of biological chemistry, Vol. 284, Issue 9, pp. 5807-18, 2009 (PubMed).

Wan, Qin, Songyang et al.: "OB fold-containing protein 1 (OBFC1), a human homolog of yeast Stn1, associates with TPP1 and is implicated in telomere length regulation." in: The Journal of biological chemistry, Vol. 284, Issue 39, pp. 26725-31, 2009 (PubMed).

Miyake, Nakamura, Nabetani et al.: "RPA-like mammalian Ctc1-Stn1-Ten1 complex binds to single-stranded DNA and protects telomeres independently of the Pot1 pathway." in: Molecular cell, Vol. 36, Issue 2, pp. 193-206, 2009 (PubMed).

Levy, Neuhausen, Hunt et al.: "Genome-wide association identifies OBFC1 as a locus involved in human leukocyte telomere biology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 20, pp. 9293-8, 2010 (PubMed).

Validation Images
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