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Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1) (AA 1-272) antibody

Details for Product No. ABIN396077, Supplier: Log in to see
Antigen
  • 0610009H20Rik
  • 2310057J23Rik
  • AAF-44
  • AAF44
  • AI413458
  • ATSTN1
  • bA541N10.2
  • F10K1.17
  • F10K1_17
  • RP11-541N10.2
  • RPA-32
  • STN1
  • zgc:64174
Epitope
AA 1-272
19
16
8
6
4
2
2
1
Reactivity
Human
43
2
Host
Mouse
26
19
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
40
24
7
6
2
1
1
1
Supplier
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Immunogen OBFC1 (AAH17400, 1 a.a. ~ 272 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G12-1B7
Isotype IgG2a kappa
Specificity OBFC1 (AAH17400, 1 a.a. ~ 272 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name OBFC1 (OBFC1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 42119 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106, MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1) (AA 1-272) antibody (ABIN396077) OBFC1 monoclonal antibody (M01), clone 3G12-1B7 Western Blot analysis of OBFC1 expres...
Background publications Levy, Neuhausen, Hunt, Kimura, Hwang, Chen, Bis, Fitzpatrick, Smith, Johnson, Gardner, Srinivasan, Schork, Rotter, Herbig, Psaty, Sastrasinh, Murray, Vasan, Province, Glazer, Lu, Cao, Kronmal, Mangino, Soranzo, Spector, Berenson, Aviv: "Genome-wide association identifies OBFC1 as a locus involved in human leukocyte telomere biology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 20, pp. 9293-8, 2010 (PubMed).

Miyake, Nakamura, Nabetani, Shimamura, Tamura, Yonehara, Saito, Ishikawa: "RPA-like mammalian Ctc1-Stn1-Ten1 complex binds to single-stranded DNA and protects telomeres independently of the Pot1 pathway." in: Molecular cell, Vol. 36, Issue 2, pp. 193-206, 2009 (PubMed).

Wan, Qin, Songyang, Liu: "OB fold-containing protein 1 (OBFC1), a human homolog of yeast Stn1, associates with TPP1 and is implicated in telomere length regulation." in: The Journal of biological chemistry, Vol. 284, Issue 39, pp. 26725-31, 2009 (PubMed).

Casteel, Zhuang, Zeng, Perrino, Boss, Goulian, Pilz: "A DNA polymerase-{alpha}{middle dot}primase cofactor with homology to replication protein A-32 regulates DNA replication in mammalian cells." in: The Journal of biological chemistry, Vol. 284, Issue 9, pp. 5807-18, 2009 (PubMed).

Vasan, Larson, Aragam, Wang, Mitchell, Kathiresan, Newton-Cheh, Vita, Keyes, ODonnell, Levy, Benjamin: "Genome-wide association of echocardiographic dimensions, brachial artery endothelial function and treadmill exercise responses in the Framingham Heart Study." in: BMC medical genetics, Vol. 8 Suppl 1, pp. S2, 2007 (PubMed).