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Elastase 3A (ELA3A) (AA 16-271) antibody

Details for Product No. ABIN396084
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Antigen
Synonyms ELA3, ELA3A, ela3a
Epitope
AA 16-271
(7), (6), (4), (2), (1)
Reactivity
Human
(22)
Host
Mouse
(11), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(22), (15), (13), (4), (3), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN396084
450.00 $
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Immunogen ELA3A (AAH07028, 16 a.a. ~ 271 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G4
Isotype IgG2a kappa
Specificity ELA3A (AAH07028, 16 a.a. ~ 271 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ELA3A
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 29475 DA
Gene ID 12406
Research Area Metabolism, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Elastase 3A (ELA3A) (AA 16-271) antibody Immunoperoxidase of monoclonal antibody to ELA3A on formalin-fixed paraffin-embedded human pancreas (antibody concentration 3 µg/mL). Detection limit for recombinant GST tagged ELA3A is approximately 1 ng/mL as a capture antibody.
anti-Elastase 3A (ELA3A) (AA 16-271) antibody (2) anti-Elastase 3A (ELA3A) (AA 16-271) antibody (Image 2)
Background publications Maruyama, Sugano: "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides." in: Gene, Vol. 138, Issue 1-2, pp. 171-4, 1994 (PubMed).

Suzuki, Yoshitomo-Nakagawa, Maruyama et al.: "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library." in: Gene, Vol. 200, Issue 1-2, pp. 149-56, 1997 (PubMed).

Shimada, Yamaguchi, Takahashi et al.: "Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells." in: International journal of molecular medicine, Vol. 10, Issue 5, pp. 599-603, 2002 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Validation Images
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