Elastase 3A (ELA3A) antibody

Antigen: Elastase 3A (ELA3A)

Synonyms: ELA3, ELA3A, ela3a

Clonality: Monoclonal (3G4)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396084

Additional Information

Alternative name: ELA3A

Gene ID: 10136

Immunogen: ELA3A (AAH07028, 16 a.a. ~ 271 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3G4

Description: Other names: ELA3 elastase 3A, pancreatic

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ELA3A (AAH07028, 16 a.a. ~ 271 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 29475 DA

Comments:

Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to ELA3A ( Cat # AT1889a ) on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml] Detection limit for recombinant GST tagged ELA3A is approximately 1ng/ml as a capture antibody.

anti-Elastase 3A (ELA3A) antibody (Image 2)

Publications

Product: Maruyama, Sugano: "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides." in: Gene, Vol. 138, Issue 1-2, pp. 171-4, 1994 (PubMed).

Suzuki, Yoshitomo-Nakagawa, Maruyama et al.: "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library." in: Gene, Vol. 200, Issue 1-2, pp. 149-56, 1997 (PubMed).

Shimada, Yamaguchi, Takahashi et al.: "Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells." in: International journal of molecular medicine, Vol. 10, Issue 5, pp. 599-603, 2002 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).