Homogentisate 1,2-Dioxygenase (HGD) (Met10) antibody

Antigen: Homogentisate 1,2-Dioxygenase (HGD)

Synonyms: AKU, HGO, FLJ94126, Hgo, aku, CG4779, DmelCG4779, hgo, MGC82288, HGD, DKFZp469K2032

Binding Site: Met10

Clonality: Monoclonal (3G4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN396085

Additional Information

Alternative name: HGD

Gene ID: 3081

UniProt: NP_000178

Immunogen: HGD (NP_000178, 377 a.a. ~ 446 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 3G4

Description: Other names: AKU, HGO homogentisate 1,2-dioxygenase (homogentisate oxidase)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: HGD (NP_000178, 377 a.a. ~ 446 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 49973 DA

Comments:

Background: This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged HGD is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Abdulrazzaq, Ibrahim, Al-Khayat et al.: "R58fs mutation in the HGD gene in a family with alkaptonuria in the UAE." in: Annals of human genetics, Vol. 73, Issue 1, pp. 125-30, 2009 (PubMed).

Vilboux, Kayser, Introne et al.: "Mutation spectrum of homogentisic acid oxidase (HGD) in alkaptonuria." in: Human mutation, Vol. 30, Issue 12, pp. 1611-9, 2009 (PubMed).

Joslyn, Ravindranathan, Brush et al.: "Human variation in alcohol response is influenced by variation in neuronal signaling genes." in: Alcoholism, clinical and experimental research, Vol. 34, Issue 5, pp. 800-12, 2010 (PubMed).

in: (PubMed).