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Secretory Carrier Membrane Protein 3 (SCAMP3) (AA 70-170) antibody

Details for Product No. ABIN396090, Supplier: Login to see
Antigen
  • scamp3
  • MGC84585
  • SCAMP3
  • MGC97532
  • C1orf3
  • Sc3
  • TU52
  • F11P17.4
  • F11P17_4
  • secretory carrier 3
Epitope
AA 70-170
5
5
3
2
2
1
1
Reactivity
Human
35
3
2
1
1
1
1
1
1
1
Host
Mouse
27
6
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
4
Application
ELISA, Western Blotting (WB)
32
18
15
4
3
2
1
1
1
Supplier
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Immunogen SCAMP3 (NP_005689, 70 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G4
Isotype IgG1 lambda
Specificity SCAMP3 (NP_005689, 70 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SCAMP3 (SCAMP3 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 38287 DA
Gene ID 12406
NCBI Accession NP_005689
Research Area Cell Structure, Cell Cycle
Pathways
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene product belongs to the SCAMP family of proteins which are secretory carrier membrane proteins. They function as carriers to the cell surface in post-golgi recycling pathways. Different family members are highly related products of distinct genes, and are usually expressed together. These findings suggest that the SCAMPs may function at the same site during vesicular transport rather than in separate pathways. Two transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-Secretory Carrier Membrane Protein 3 (SCAMP3) (AA 70-170) antibody (ABIN396090) Detection limit for recombinant GST tagged SCAMP3 is approximately 0.3 ng/mL as a cap...
Background publications Mota, Ramsden, Liu et al.: "SCAMP3 is a component of the Salmonella-induced tubular network and reveals an interaction between bacterial effectors and post-Golgi trafficking." in: Cellular microbiology, Vol. 11, Issue 8, pp. 1236-53, 2009 (PubMed).

Aoh, Castle, Hubbard et al.: "SCAMP3 negatively regulates epidermal growth factor receptor degradation and promotes receptor recycling." in: Molecular biology of the cell, Vol. 20, Issue 6, pp. 1816-32, 2009 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).