ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8) antibody

Antigen: ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8)

Synonyms: HI, SUR, HHF1, MRP8, PHHI, SUR1, ABC36, HRINS, TNDM2, Sur, Sur1

Clonality: Monoclonal (3G5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396093

Additional Information

Alternative name: ABCC8

Gene ID: 6833

UniProt: NP_000343

Immunogen: ABCC8 (NP_000343, 611 a.a. ~ 711 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3G5

Description: Other names: ABC36, HI, HRINS, MRP8, PHHI, SUR, SUR1 ATP-binding cassette, sub-family C (CFTR/MRP), member 8

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ABCC8 (NP_000343, 611 a.a. ~ 711 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 177022 DA

Comments:

Background: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternative splicing of this gene has been observed, however, the transcript variants have not been fully described.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged ABCC8 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Ruchat, Vohl, Weisnagel et al.: "Combining genetic markers and clinical risk factors improves the risk assessment of impaired glucose metabolism." in: Annals of medicine, Vol. 42, Issue 3, pp. 196-206, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Jablonski, McAteer, de Bakker et al.: "Common variants in 40 genes assessed for diabetes incidence and response to metformin and lifestyle intervention in the diabetes prevention program." in: Diabetes, Vol. 59, Issue 10, pp. 2672-81, 2010 (PubMed).