N-Acetylneuraminic Acid Synthase (NANS) antibody

Antigen: N-Acetylneuraminic Acid Synthase (NANS)

Synonyms: SAS, MGC84329, NANS, MGC137759

Clonality: Monoclonal (3G6)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396101

Additional Information

Alternative name: NANS

Gene ID: 54187

UniProt: NP_061819

Immunogen: NANS (NP_061819, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3G6

Description: Other names: SAS N-acetylneuraminic acid synthase (sialic acid synthase)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: NANS (NP_061819, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 40308 DA

Comments:

Background: This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively, however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

NANS monoclonal antibody (M01), clone 3G6 Western Blot analysis of NANS expression in HeLa ( Cat # L013V1 ). Detection limit for recombinant GST tagged NANS is approximately 0.03ng/ml as a capture antibody.

anti-N-Acetylneuraminic Acid Synthase (NANS) antibody (Image 2)

Publications

Product: Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Humphray, Oliver, Hunt et al.: "DNA sequence and analysis of human chromosome 9." in: Nature, Vol. 429, Issue 6990, pp. 369-74, 2004 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Hao, Balagurumoorthy, Sarilla et al.: "Cloning, expression, and characterization of sialic acid synthases." in: Biochemical and biophysical research communications, Vol. 338, Issue 3, pp. 1507-14, 2005 (PubMed).

Hamada, Ito, Abe et al.: "Solution structure of the antifreeze-like domain of human sialic acid synthase." in: Protein science : a publication of the Protein Society, Vol. 15, Issue 5, pp. 1010-6, 2006 (PubMed).