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Details for Product No. ABIN396101

N-Acetylneuraminic Acid Synthase (NANS) antibody

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Antigen
Synonyms SAS, MGC84329
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN396101
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen NANS (NP_061819, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G6
Isotype IgG2a kappa
Specificity NANS (NP_061819, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name NANS
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 40308 DA
Gene ID 12406
UniProt NP_061819
Research Area Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively, however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Humphray, Oliver, Hunt et al.: "DNA sequence and analysis of human chromosome 9." in: Nature, Vol. 429, Issue 6990, pp. 369-74, 2004 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Hao, Balagurumoorthy, Sarilla et al.: "Cloning, expression, and characterization of sialic acid synthases." in: Biochemical and biophysical research communications, Vol. 338, Issue 3, pp. 1507-14, 2005 (PubMed).

Hamada, Ito, Abe et al.: "Solution structure of the antifreeze-like domain of human sialic acid synthase." in: Protein science : a publication of the Protein Society, Vol. 15, Issue 5, pp. 1010-6, 2006 (PubMed).

Alternatives for antigen "N-Acetylneuraminic Acid Synthase (NANS)", type "Antibodies"
Hosts (10), (8)
Reactivities (18), (2), (2)
Applications (17), (9), (2), (2), (1)
Epitopes (2), (2), (1), (1), (1), (1)
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