Decorin (DCN) antibody

Antigen: Decorin (DCN)

Synonyms: CSCD, PG40, PGII, PGS2, DSPG2, SLRR1B, MGC94682, DCN, MGC127810

Clonality: Monoclonal (3H4-1F4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396139

Additional Information

Alternative name: DCN

Gene ID: 1634

Immunogen: DCN (AAH05322, 1 a.a. ~ 360 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 3H4-1F4

Description: Other names: DSPG2, PG40, PGII, PGS2, SLRR1B decorin

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DCN (AAH05322, 1 a.a. ~ 360 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 39747 DA

Comments:

Background: The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to DCN ( Cat # AT1716a ) on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3 ug/ml] Detection limit for recombinant GST tagged DCN is approximately 1ng/ml as a capture antibody.

anti-Decorin (DCN) antibody (Image 2)

Publications

Product: Dimasi, Burdon, Hewitt et al.: "Candidate gene study to investigate the genetic determinants of normal variation in central corneal thickness." in: Molecular vision, Vol. 16, pp. 562-9, 2010 (PubMed).

Mohan, Gupta, Mehan et al.: "Decorin transfection suppresses profibrogenic genes and myofibroblast formation in human corneal fibroblasts." in: Experimental eye research, Vol. 91, Issue 2, pp. 238-45, 2010 (PubMed).

Swan, Murthi, Rajaraman et al.: "Decorin expression is decreased in human idiopathic fetal growth restriction." in: Reproduction, fertility, and development, Vol. 22, Issue 6, pp. 949-55, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).