Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN396141

Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms MES, HUMNDME, D9Ertd267e, Mdh-1, Mod-1, Mod1, MOD1, ME1, zgc:153079
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN396141
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 2 to 3 Business Days
Immunogen ME1 (AAH25246, 1 a.a. ~ 573 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3H5
Isotype IgG1 kappa
Specificity ME1 (AAH25246, 1 a.a. ~ 573 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ME1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 64150 DA
Gene ID 12406
Research Area Metabolism, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Restrictions For Research Use only
Concentration 1mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Lu, Dollé, Imholz et al.: "Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations." in: Journal of lipid research, Vol. 49, Issue 12, pp. 2582-9, 2008 (PubMed).

Heart, Cline, Collis et al.: "Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion." in: American journal of physiology. Endocrinology and metabolism, Vol. 296, Issue 6, pp. E1354-62, 2009 (PubMed).

Hsieh, Chen, Hung: "Functional roles of the tetramer organization of malic enzyme." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18096-105, 2009 (PubMed).

Hsieh, Su, Ho et al.: "Long-range interaction between the enzyme active site and a distant allosteric site in the human mitochondrial NAD(P)+-dependent malic enzyme." in: Archives of biochemistry and biophysics, Vol. 487, Issue 1, pp. 19-27, 2009 (PubMed).

Alternatives for antigen "Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1)", type "Antibodies"
Hosts (36), (5)
Reactivities (39), (25), (25), (13), (13), (12), (1), (1), (1)
Applications (32), (21), (10), (6), (3), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (4), (2), (2), (1), (1)
Beacon