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BLMH antibody (Bleomycin Hydrolase) (AA 356-455)

Details for Product anti-BLMH Antibody No. ABIN396175, Supplier: Login to see
Antigen
  • AI035728
  • Bh
  • Bmh
  • wu:fb13c01
  • zgc:66261
  • bh
  • bmh
  • BH
  • BMH
Epitope
AA 356-455
10
6
6
5
3
3
3
3
2
2
2
2
2
2
1
1
Reactivity
Human
59
33
32
1
1
1
1
1
Host
Mouse
60
18
1
Clonality (Clone)
Monoclonal ()
Conjugate
This BLMH antibody is un-conjugated
7
6
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
47
29
11
10
7
7
6
4
3
2
Supplier
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Immunogen BLMH (NP_000377, 356 a.a. ~ 455 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4A2
Isotype IgG2a kappa
Specificity BLMH (NP_000377, 356 a.a. ~ 455 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BLMH (BLMH Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 52562 DA
Gene ID 12406
NCBI Accession NP_000377
Research Area Cancer, Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution, however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-BLMH antibody (Bleomycin Hydrolase) (AA 356-455) (ABIN396175) BLMH monoclonal antibody (M02), clone 4A2. Western Blot analysis of BLMH expression i...
Immunohistochemistry (IHC) image for anti-BLMH antibody (Bleomycin Hydrolase) (AA 356-455) (ABIN396175) anti-Bleomycin Hydrolase (BLMH) (AA 356-455) antibody (Image 2)
Background publications Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

de Haas, Zwart, Meijer et al.: "Variation in bleomycin hydrolase gene is associated with reduced survival after chemotherapy for testicular germ cell cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 11, pp. 1817-23, 2008 (PubMed).

Maffei, Carbone, Angelini et al.: "Micronuclei frequency induced by bleomycin in human peripheral lymphocytes: correlating BLHX polymorphism with mutagen sensitivity." in: Mutation research, Vol. 639, Issue 1-2, pp. 20-6, 2008 (PubMed).

Llorca, Rodríguez-Rodríguez, Dierssen-Sotos et al.: "Meta-analysis of genetic variability in the beta-amyloid production, aggregation and degradation metabolic pathways and the risk of Alzheimer's disease." in: Acta neurologica Scandinavica, Vol. 117, Issue 1, pp. 1-14, 2007 (PubMed).

Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network. ..." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).