Bleomycin Hydrolase (BLMH) (AA 356-455) antibody

Details for Product No. ABIN396175
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms AI035728, Bh, Bmh, wu:fb13c01, zgc:66261, bh, bmh, BH, BMH
Epitope
AA 356-455
(3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(38), (22), (20), (13), (13), (12), (1), (1), (1)
Host
Mouse
(35), (10), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(34), (14), (10), (9), (8), (7), (6), (4), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN396175
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen BLMH (NP_000377, 356 a.a. ~ 455 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4A2
Isotype IgG2a kappa
Specificity BLMH (NP_000377, 356 a.a. ~ 455 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BLMH
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 52562 DA
Gene ID 12406
NCBI Accession NP_000377
Research Area Cancer, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution, however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Bleomycin Hydrolase (BLMH) (AA 356-455) antibody BLMH monoclonal antibody (M02), clone 4A2. Western Blot analysis of BLMH expression in K-562. Detection limit for recombinant GST tagged BLMH is approximately 0.3 ng/mL as a capture antibody.
anti-Bleomycin Hydrolase (BLMH) (AA 356-455) antibody (2) anti-Bleomycin Hydrolase (BLMH) (AA 356-455) antibody (Image 2)
Background publications Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Llorca, Rodríguez-Rodríguez, Dierssen-Sotos et al.: "Meta-analysis of genetic variability in the beta-amyloid production, aggregation and degradation metabolic pathways and the risk of Alzheimer's disease." in: Acta neurologica Scandinavica, Vol. 117, Issue 1, pp. 1-14, 2007 (PubMed).

Maffei, Carbone, Angelini et al.: "Micronuclei frequency induced by bleomycin in human peripheral lymphocytes: correlating BLHX polymorphism with mutagen sensitivity." in: Mutation research, Vol. 639, Issue 1-2, pp. 20-6, 2008 (PubMed).

de Haas, Zwart, Meijer et al.: "Variation in bleomycin hydrolase gene is associated with reduced survival after chemotherapy for testicular germ cell cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 11, pp. 1817-23, 2008 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Validation Images
Did you look for something else?
back to top