Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5) (AA 319-401) antibody

Details for Product No. ABIN396189
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Antigen
Synonyms ENTPD5, CD39L4, NTPDase-5, PCPH, AI196558, AI987697, Cd39l4, ER-UDPase, NTPDase5, Pcph, mNTPase, cph
Epitope
AA 319-401
(7), (7), (7), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(43), (13), (13), (12)
Host
Mouse
(30), (12), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(32), (16), (10), (7), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN396189
450.00 $
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Immunogen ENTPD5 (NP_001240, 319 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4A5
Isotype IgG2b kappa
Specificity ENTPD5 (NP_001240, 319 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ENTPD5
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 47517 DA
Gene ID 12406
NCBI Accession NP_001240
Research Area Metabolism, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is similar to E-type nucleotidases (NTPases)/ecto-ATPase/apyrases. NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase-conserved regions which is characteristic of NTPases.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5) (AA 319-401) antibody ENTPD5 monoclonal antibody (M07), clone 4A5 Western Blot analysis of ENTPD5 expression in HepG2. Detection limit for recombinant GST tagged ENTPD5 is approximately 0.3 ng/mL as a capture antibody.
anti-Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5) (AA 319-401) antibody (2) anti-Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5) (AA 319-401) antibody (Image 2)
Background publications Regadera, Blánquez, González-Peramato et al.: "PCPH expression is an early event in the development of testicular germ cell tumors." in: International journal of oncology, Vol. 28, Issue 3, pp. 595-604, 2006 (PubMed).

Villar, Arenas, MacCarthy et al.: "PCPH/ENTPD5 expression enhances the invasiveness of human prostate cancer cells by a protein kinase C delta-dependent mechanism." in: Cancer research, Vol. 67, Issue 22, pp. 10859-68, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Villar, Quadri, Song et al.: "PCPH/ENTPD5 expression confers to prostate cancer cells resistance against cisplatin-induced apoptosis through protein kinase Calpha-mediated Bcl-2 stabilization." in: Cancer research, Vol. 69, Issue 1, pp. 102-10, 2009 (PubMed).

Fransen, Visschedijk, van Sommeren et al.: "Analysis of SNPs with an effect on gene expression identifies UBE2L3 and BCL3 as potential new risk genes for Crohn's disease." in: Human molecular genetics, Vol. 19, Issue 17, pp. 3482-8, 2010 (PubMed).

Validation Images
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