Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5) antibody

Antigen: Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5)

Synonyms: PCPH, CD39L4, MGC163357, MGC163359, NTPDase-5, Pcph, Cd39l4, mNTPase, AI196558, AI987697, NTPDase5, ER-UDPase, ENTPD5, entpd5

Clonality: Monoclonal (4A5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396189

Additional Information

Alternative name: ENTPD5

Gene ID: 957

UniProt: NP_001240

Immunogen: ENTPD5 (NP_001240, 319 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 4A5

Description: Other names: CD39L4, NTPDase5, PCPH ectonucleoside triphosphate diphosphohydrolase 5

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ENTPD5 (NP_001240, 319 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 47517 DA

Comments:

Background: The protein encoded by this gene is similar to E-type nucleotidases (NTPases)/ecto-ATPase/apyrases. NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase-conserved regions which is characteristic of NTPases.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

ENTPD5 monoclonal antibody (M07), clone 4A5 Western Blot analysis of ENTPD5 expression in HepG2 ( Cat # L019V1 ). Detection limit for recombinant GST tagged ENTPD5 is approximately 0.3ng/ml as a capture antibody.

anti-Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5) antibody (Image 2)

Publications

Product: Regadera, Blánquez, González-Peramato et al.: "PCPH expression is an early event in the development of testicular germ cell tumors." in: International journal of oncology, Vol. 28, Issue 3, pp. 595-604, 2006 (PubMed).

Villar, Arenas, MacCarthy et al.: "PCPH/ENTPD5 expression enhances the invasiveness of human prostate cancer cells by a protein kinase C delta-dependent mechanism." in: Cancer research, Vol. 67, Issue 22, pp. 10859-68, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Villar, Quadri, Song et al.: "PCPH/ENTPD5 expression confers to prostate cancer cells resistance against cisplatin-induced apoptosis through protein kinase Calpha-mediated Bcl-2 stabilization." in: Cancer research, Vol. 69, Issue 1, pp. 102-10, 2009 (PubMed).

Fransen, Visschedijk, van Sommeren et al.: "Analysis of SNPs with an effect on gene expression identifies UBE2L3 and BCL3 as potential new risk genes for Crohn's disease." in: Human molecular genetics, Vol. 19, Issue 17, pp. 3482-8, 2010 (PubMed).