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Oculocerebrorenal Syndrome of Lowe (OCRL) (AA 146-240) antibody

Details for Product No. ABIN396192, Supplier: Log in to see
Antigen
  • OCRL
  • wu:fi09g03
  • zgc:152864
  • locr
  • nphl2
  • ocrl1
  • inpp5f
  • INPP5F
  • LOCR
  • NPHL2
  • OCRL-1
  • OCRL1
  • 9530014D17Rik
  • BB143339
Epitope
AA 146-240
17
5
5
3
3
3
2
1
1
1
1
1
1
1
1
Reactivity
Human
52
8
5
2
Host
Mouse
30
22
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
47
18
18
6
6
2
1
1
1
1
1
Supplier
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Immunogen OCRL (NP_000267, 146 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4A6
Isotype IgG2a kappa
Specificity OCRL (NP_000267, 146 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name OCRL (OCRL Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 104205 DA
Gene ID 12406
NCBI Accession NP_000267
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a phosphatase enzyme that is involved in actin polymerization and is found in the trans-Golgi network. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Oculocerebrorenal Syndrome of Lowe (OCRL) (AA 146-240) antibody (ABIN396192) Western blot analysis of OCRL over-expressed 293 cell line, cotransfected with OCRL V...
Immunohistochemistry (IHC) image for anti-Oculocerebrorenal Syndrome of Lowe (OCRL) (AA 146-240) antibody (ABIN396192) anti-Oculocerebrorenal Syndrome of Lowe (OCRL) (AA 146-240) antibody (Image 2)
Background publications Swan, Tomasini, Pirruccello et al.: "Two closely related endocytic proteins that share a common OCRL-binding motif with APPL1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 8, pp. 3511-6, 2010 (PubMed).

Coutton, Monnier, Rendu et al.: "Development of a multiplex ligation-dependent probe amplification (MLPA) assay for quantification of the OCRL1 gene." in: Clinical biochemistry, Vol. 43, Issue 6, pp. 609-14, 2010 (PubMed).

Cui, Guerriero, Szalinski et al.: "OCRL1 function in renal epithelial membrane traffic." in: American journal of physiology. Renal physiology, Vol. 298, Issue 2, pp. F335-45, 2010 (PubMed).

Coon, Mukherjee, Hanna et al.: "Lowe syndrome patient fibroblasts display Ocrl1-specific cell migration defects that cannot be rescued by the homologous Inpp5b phosphatase." in: Human molecular genetics, Vol. 18, Issue 23, pp. 4478-91, 2009 (PubMed).

Tosetto, Addis, Caridi et al.: "Locus heterogeneity of Dent's disease: OCRL1 and TMEM27 genes in patients with no CLCN5 mutations." in: Pediatric nephrology (Berlin, Germany), Vol. 24, Issue 10, pp. 1967-73, 2009 (PubMed).