3-hydroxybutyrate Dehydrogenase, Type 1 (BDH1) antibody

Antigen: 3-hydroxybutyrate Dehydrogenase, Type 1 (BDH1)

Synonyms: BDH, SDR9C1, MGC2723, MGC4347, MGC9788, Bdh, AI327223, 2310032J20Rik, MGC94818, MGC128244, zgc:163053

Clonality: Monoclonal (4B3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396225

Additional Information

Alternative name: BDH1

Gene ID: 622

Immunogen: BDH1 (AAH11964, 1 a.a. ~ 344 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4B3

Description: Other names: BDH, MGC2723, MGC4347, MGC9788 3-hydroxybutyrate dehydrogenase, type 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BDH1 (AAH11964, 1 a.a. ~ 344 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 38157 DA

Comments:

Background: This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Metabolism

Restrictions: For Research Use only

Images

BDH1 monoclonal antibody (M03), clone 4B3. Western Blot analysis of BDH1 expression in Raw 264.7 ( Cat # L024V1 ).

Publications

Product: Loeb-Hennard, McIntyre: "(R)-3-hydroxybutyrate dehydrogenase: selective phosphatidylcholine binding by the C-terminal domain." in: Biochemistry, Vol. 39, Issue 39, pp. 11928-38, 2000 (PubMed).

Xu, Huang, Xu et al.: "Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 26, pp. 15089-94, 2001 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Persson, Kallberg, Bray et al.: "The SDR (short-chain dehydrogenase/reductase and related enzymes) nomenclature initiative." in: Chemico-biological interactions, Vol. 178, Issue 1-3, pp. 94-8, 2009 (PubMed).