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ICT1 antibody (Immature Colon Carcinoma Transcript 1) (AA 107-207)

Details for Product anti-ICT1 Antibody No. ABIN396263, Supplier: Log in to see
Antigen
  • 1110001A02Rik
  • 1110002E03Rik
  • DS-1
  • DS1
  • ICT1
Epitope
AA 107-207
7
4
4
4
3
3
2
2
1
1
Reactivity
Human
54
20
20
12
1
Host
Mouse
34
20
Clonality (Clone)
Monoclonal ()
Conjugate
This ICT1 antibody is un-conjugated
4
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
38
23
11
4
4
1
1
1
1
Supplier
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Immunogen ICT1 (NP_001536, 107 a.a. ~ 207 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4C10
Isotype IgG2a kappa
Specificity ICT1 (NP_001536, 107 a.a. ~ 207 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ICT1 (ICT1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 23630 DA
Gene ID 12406
NCBI Accession NP_001536
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is ICT1 whose mRNA and protein were more highly expressed in undifferentiated than in differentiated cells.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-ICT1 antibody (Immature Colon Carcinoma Transcript 1) (AA 107-207) (ABIN396263) ICT1 monoclonal antibody (M06), clone 4C10 Western Blot analysis of ICT1 expression i...
Background publications Antonicka, Ostergaard, Sasarman, Weraarpachai, Wibrand, Pedersen, Rodenburg, van der Knaap, Smeitink, Chrzanowska-Lightowlers, Shoubridge: "Mutations in C12orf65 in patients with encephalomyopathy and a mitochondrial translation defect." in: American journal of human genetics, Vol. 87, Issue 1, pp. 115-22, 2010 (PubMed).

Richter, Rorbach, Pajak, Smith, Wessels, Huynen, Smeitink, Lightowlers, Chrzanowska-Lightowlers: "A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome." in: The EMBO journal, Vol. 29, Issue 6, pp. 1116-25, 2010 (PubMed).

Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Suzuki, Yoshitomo-Nakagawa, Maruyama, Suyama, Sugano: "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library." in: Gene, Vol. 200, Issue 1-2, pp. 149-56, 1997 (PubMed).