Galactose-1-Phosphate Uridylyltransferase (GALT) antibody

Antigen: Galactose-1-Phosphate Uridylyltransferase (GALT)

Synonyms: MGC112778, GALT, AW553376

Clonality: Monoclonal (4C11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396266

Additional Information

Alternative name: GALT

Gene ID: 2592

Immunogen: GALT (AAH15045, 1 a.a. ~ 380 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4C11

Description: Other names: galactose-1-phosphate uridylyltransferase

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: GALT (AAH15045, 1 a.a. ~ 380 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 43363 DA

Comments:

Background: Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of GALT expression in transfected 293T cell line by GALT monoclonal antibody (M01), clone 4C11. Lane 1: GALT transfected lysate(43 KDa). Lane 2: Non-transfected lysate. GALT monoclonal antibody (M01), clone 4C11 Western Blot analysis of GALT expression in K-562 ( Cat # L009V1 ).

anti-Galactose-1-Phosphate Uridylyltransferase (GALT) antibody (Image 2)

Publications

Product: Nijland, Hartog, Wevers et al.: "Galactose-1-phosphate uridyl transferase deficiency is not associated with Müllerian aplasia in Dutch patients." in: Journal of pediatric and adolescent gynecology, Vol. 22, Issue 4, pp. 229-31, 2009 (PubMed).

Facchiano, Marabotti: "Analysis of galactosemia-linked mutations of GALT enzyme using a computational biology approach." in: Protein engineering, design & selection : PEDS, Vol. 23, Issue 2, pp. 103-13, 2010 (PubMed).

Ounap, Joost, Temberg et al.: "Classical galactosemia in Estonia: selective neonatal screening, incidence, and genotype/phenotype data of diagnosed patients." in: Journal of inherited metabolic disease, Vol. 33, Issue 2, pp. 175-6, 2010 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Ko, Chang, Song et al.: "Molecular and biochemical characterization of the GALT gene in Korean patients with galactose-1-phosphate uridyltransferase deficiency." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 411, Issue 19-20, pp. 1506-10, 2010 (PubMed).