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beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3) antibody
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||B3GAT3 (NP_036332, 236 a.a. ~ 336 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: GLCATI, GlcATIbeta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||B3GAT3 (NP_036332, 236 a.a. ~ 336 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||37122 DA|
Background: The protein encoded by this gene is a member of the glucuronyltransferase gene family, enzymes that exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product catalyzes the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Metabolism, Cell Structure|
|Restrictions||For Research Use only|
|Western Blot analysis of B3GAT3 expression in transfected 293T cell line by B3GAT3 monoclonal antibody (M01), clone 4C12. Lane 1: B3GAT3 transfected lysate(37.1 KDa). Lane 2: Non-transfected lysate.|
Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).
Gulberti, Lattard, Fondeur et al.: "Phosphorylation and sulfation of oligosaccharide substrates critically influence the activity of human beta1,4-galactosyltransferase 7 (GalT-I) and beta1,3-glucuronosyltransferase I (GlcAT-I) involved in the biosynthesis of the glycosaminoglycan-protein l" in: The Journal of biological chemistry, Vol. 280, Issue 2, pp. 1417-25, 2005 (PubMed).
Venkatesan, Barré, Benani et al.: "Stimulation of proteoglycan synthesis by glucuronosyltransferase-I gene delivery: a strategy to promote cartilage repair." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 52, pp. 18087-92, 2004 (PubMed).
Fondeur-Gelinotte, Lattard, Gulberti et al.: "Molecular basis for acceptor substrate specificity of the human beta1,3-glucuronosyltransferases GlcAT-I and GlcAT-P involved in glycosaminoglycan and HNK-1 carbohydrate epitope biosynthesis, respectively." in: Glycobiology, Vol. 17, Issue 8, pp. 857-67, 2007 (PubMed).
Tone, Pedersen, Yamamoto et al.: "2-o-phosphorylation of xylose and 6-o-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage region synthesis." in: The Journal of biological chemistry, Vol. 283, Issue 24, pp. 16801-7, 2008 (PubMed).