anti-RALB antibody (V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein)) (AA 89-183)

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Antigen
Synonyms dRalb, 5730472O18Rik
Alternatives
Epitope
AA 89-183
(8), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(54), (25), (23), (3), (2)
Host
Mouse
(39), (14), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
This RALB antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(45), (13), (11), (10), (8), (7), (3), (1), (1)
Pubmed 5 references available
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Quantity 0.1 mg
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Immunogen RALB (AAH18163, 89 a.a. ~ 183 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4D1
Isotype IgG2a kappa
Specificity RALB (AAH18163, 89 a.a. ~ 183 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RALB (Ralb Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 23409 DA
Gene ID 12406
Research Area Cell Signaling, Cell Structure
Pathways Neurotrophin Signaling Pathway
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-RALB antibody (V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein)) (AA 89-183) (ABIN396308) Western Blot analysis of RALB expression in transfected 293T cell line by RALB monocl...
Immunohistochemistry (IHC) image for anti-RALB antibody (V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein)) (AA 89-183) (ABIN396308) anti-V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein) (Ralb) (AA 89-183) antibody (Image 2)
Background publications Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Prasannan, Fenwick, Campbell et al.: "1H, 13C and 15N resonance assignments for the small G protein RalB in its active conformation." in: Biomolecular NMR assignments, Vol. 1, Issue 1, pp. 147-9, 2009 (PubMed).

Aziziyeh, Li, Pape et al.: "Dual regulation of lysophosphatidic acid (LPA1) receptor signalling by Ral and GRK." in: Cellular signalling, Vol. 21, Issue 7, pp. 1207-17, 2009 (PubMed).

Fenwick, Prasannan, Campbell et al.: "Solution structure and dynamics of the small GTPase RalB in its active conformation: significance for effector protein binding." in: Biochemistry, Vol. 48, Issue 10, pp. 2192-206, 2009 (PubMed).

Cascone, Selimoglu, Ozdemir et al.: "Distinct roles of RalA and RalB in the progression of cytokinesis are supported by distinct RalGEFs." in: The EMBO journal, Vol. 27, Issue 18, pp. 2375-87, 2008 (PubMed).

Catalog No. ABIN396308
$450.00
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