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RALB antibody (V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein)) (AA 89-183)

Details for Product anti-Ralb Antibody No. ABIN396308, Supplier: Log in to see
Antigen
  • dRalb
  • 5730472O18Rik
Alternatives
anti-Human RALB antibody for Western Blotting
Epitope
AA 89-183
8
4
4
3
2
1
1
1
1
1
1
Reactivity
Human
57
27
25
3
1
Host
Mouse
43
13
2
Clonality (Clone)
Monoclonal ()
Conjugate
This RALB antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
45
13
11
9
8
8
2
1
Supplier
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Immunogen RALB (AAH18163, 89 a.a. ~ 183 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4D1
Isotype IgG2a kappa
Specificity RALB (AAH18163, 89 a.a. ~ 183 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RALB (Ralb Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 23409 DA
Gene ID 12406
Research Area Cell Signaling, Cell Structure
Pathways Neurotrophin Signaling Pathway
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-RALB antibody (V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein)) (AA 89-183) (ABIN396308) Western Blot analysis of RALB expression in transfected 293T cell line by RALB monocl...
Immunohistochemistry (IHC) image for anti-RALB antibody (V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein)) (AA 89-183) (ABIN396308) anti-V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein) (Ralb) (AA 89-183) antibody (Image 2)
Background publications Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Prasannan, Fenwick, Campbell et al.: "1H, 13C and 15N resonance assignments for the small G protein RalB in its active conformation." in: Biomolecular NMR assignments, Vol. 1, Issue 1, pp. 147-9, 2009 (PubMed).

Aziziyeh, Li, Pape et al.: "Dual regulation of lysophosphatidic acid (LPA1) receptor signalling by Ral and GRK." in: Cellular signalling, Vol. 21, Issue 7, pp. 1207-17, 2009 (PubMed).

Fenwick, Prasannan, Campbell et al.: "Solution structure and dynamics of the small GTPase RalB in its active conformation: significance for effector protein binding." in: Biochemistry, Vol. 48, Issue 10, pp. 2192-206, 2009 (PubMed).

Cascone, Selimoglu, Ozdemir et al.: "Distinct roles of RalA and RalB in the progression of cytokinesis are supported by distinct RalGEFs." in: The EMBO journal, Vol. 27, Issue 18, pp. 2375-87, 2008 (PubMed).