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NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 7, 18kDa (NDUFB7) (AA 38-138) antibody

Details for Product No. ABIN396333, Supplier: Login to see
Antigen
  • 1110002H15Rik
  • B18
  • CI-B18
Epitope
AA 38-138
3
2
2
2
2
1
1
Reactivity
Human
33
5
5
Host
Mouse
28
5
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
4
Application
Western Blotting (WB)
28
20
3
2
2
1
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Immunogen NDUFB7 (NP_004137, 38 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4D4
Isotype IgG2a kappa
Specificity NDUFB7 (NP_004137, 38 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name NDUFB7 (NDUFB7 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 16402 DA
Gene ID 12406
NCBI Accession NP_004137
Research Area Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 7, 18kDa (NDUFB7) (AA 38-138) antibody (ABIN396333) Western blot analysis of NDUFB7 over-expressed 293 cell line, cotransfected with NDUF...
Immunohistochemistry (IHC) image for anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 7, 18kDa (NDUFB7) (AA 38-138) antibody (ABIN396333) anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 7, 18kDa (NDUFB7) (AA 38-138) antibody (Image 2)
Background publications Saito, Kawamoto, Kamatani: "Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects." in: Journal of human genetics, Vol. 54, Issue 6, pp. 317-23, 2009 (PubMed).

Starr, Shiels, Harris et al.: "Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey." in: Mechanisms of ageing and development, Vol. 129, Issue 12, pp. 745-51, 2008 (PubMed).

Harris, Fox, Wright et al.: "A genetic association analysis of cognitive ability and cognitive ageing using 325 markers for 109 genes associated with oxidative stress or cognition." in: BMC genetics, Vol. 8, pp. 43, 2007 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Murray, Zhang, Taylor et al.: "The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification." in: The Journal of biological chemistry, Vol. 278, Issue 16, pp. 13619-22, 2003 (PubMed).

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