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NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 7, 18kDa (NDUFB7) antibody

Details for Product No. ABIN396333
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Antigen
Synonyms B18, CI-B18, 1110002H15Rik
Reactivity
Human
(15), (2), (2)
Host
Mouse
(9), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(15), (11), (2), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN396333
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen NDUFB7 (NP_004137, 38 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4D4
Isotype IgG2a kappa
Specificity NDUFB7 (NP_004137, 38 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name NDUFB7
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 16402 DA
Gene ID 12406
UniProt NP_004137
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 7, 18kDa (NDUFB7) antibody Western blot analysis of NDUFB7 over-expressed 293 cell line, cotransfected with NDUFB7 Validated Chimera RNAi used as specificity and loading control. Western Blot analysis of NDUFB7 expression in transfected 293T cell line by NDUFB7 monoclonal antibody (M01), clone 4D4. Lane 1: NDUFB7 transfected lysate(16.4 KDa). Lane 2: Non-transfected lysate.
anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 7, 18kDa (NDUFB7) antibody (2) anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 7, 18kDa (NDUFB7) antibody (Image 2)
Background publications Murray, Zhang, Taylor et al.: "The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification." in: The Journal of biological chemistry, Vol. 278, Issue 16, pp. 13619-22, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Harris, Fox, Wright et al.: "A genetic association analysis of cognitive ability and cognitive ageing using 325 markers for 109 genes associated with oxidative stress or cognition." in: BMC genetics, Vol. 8, pp. 43, 2007 (PubMed).

Starr, Shiels, Harris et al.: "Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey." in: Mechanisms of ageing and development, Vol. 129, Issue 12, pp. 745-51, 2008 (PubMed).

Saito, Kawamoto, Kamatani: "Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects." in: Journal of human genetics, Vol. 54, Issue 6, pp. 317-23, 2009 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN396333
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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