Aminoacylase 1 (ACY1) (AA 1-409) antibody

Details for Product No. ABIN396373
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Antigen
Synonyms si:zfos-226g1.1, zgc:55605, zgc:76955, acy1, acy1d, acylase, ACY1, ACY-1, ACY1D, 1110014J22Rik, Acy-1, Acy1a, Acy1b
Epitope
AA 1-409
(20), (1), (1), (1), (1)
Reactivity
Human
(63), (20), (17), (15), (12), (4), (1)
Host
Mouse
(36), (29), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(40), (27), (25), (18), (11), (10), (10), (9), (6), (2), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN396373
450.00 $
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Immunogen ACY1 (AAH00545, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4F1-B7
Isotype IgG1 kappa
Specificity ACY1 (AAH00545, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ACY1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 45885 DA
Gene ID 12406
Research Area Cancer, Metabolism, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Aminoacylase 1 (ACY1) (AA 1-409) antibody Immunofluorescence of monoclonal antibody to ACY1 on HeLa cell (antibody concentration 10 µg/mL). ACY1 monoclonal antibody (M01), clone 4F1-B7 Western Blot analysis of ACY1 expression in K-562.
anti-Aminoacylase 1 (ACY1) (AA 1-409) antibody (2) anti-Aminoacylase 1 (ACY1) (AA 1-409) antibody (Image 2)
Background publications Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Van Coster, Gerlo, Giardina et al.: "Aminoacylase I deficiency: a novel inborn error of metabolism." in: Biochemical and biophysical research communications, Vol. 338, Issue 3, pp. 1322-6, 2005 (PubMed).

Sass, Mohr, Olbrich et al.: "Mutations in ACY1, the gene encoding aminoacylase 1, cause a novel inborn error of metabolism." in: American journal of human genetics, Vol. 78, Issue 3, pp. 401-9, 2006 (PubMed).

Figueiredo, Garcia Leão, De Oliveira et al.: "The amidase activity of human tissue kallikrein is significantly lower in the urine of patients with systolic heart failure." in: Journal of cardiac failure, Vol. 12, Issue 8, pp. 653-8, 2006 (PubMed).

Lindner, Alary, Wilke et al.: "Probing the acyl-binding pocket of aminoacylase-1." in: Biochemistry, Vol. 47, Issue 14, pp. 4266-75, 2008 (PubMed).

Validation Images
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