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Aminoacylase 1 antibody (ACY1) (AA 1-409)

Details for Product anti-ACY1 Antibody No. ABIN396373, Supplier: Log in to see
Antigen
  • si:zfos-226g1.1
  • zgc:55605
  • zgc:76955
  • acy1
  • acy1d
  • acylase
  • ACY1
  • ACY-1
  • ACY1D
  • 1110014J22Rik
  • Acy-1
  • Acy1a
  • Acy1b
Epitope
AA 1-409
17
5
3
2
2
1
1
1
1
1
Reactivity
Human
75
14
11
4
4
1
Host
Mouse
43
32
4
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Aminoacylase 1 antibody is un-conjugated
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
61
30
26
25
16
15
14
5
3
2
2
2
1
1
1
1
1
1
1
Supplier
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Immunogen ACY1 (AAH00545, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4F1-B7
Isotype IgG1 kappa
Specificity ACY1 (AAH00545, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ACY1 (ACY1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 45885 DA
Gene ID 12406
Research Area Cancer, Metabolism, Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Aminoacylase 1 antibody (ACY1) (AA 1-409) (ABIN396373) Immunofluorescence of monoclonal antibody to ACY1 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-Aminoacylase 1 antibody (ACY1) (AA 1-409) (ABIN396373) anti-Aminoacylase 1 (ACY1) (AA 1-409) antibody (Image 2)
Background publications Lindner, Alary, Wilke et al.: "Probing the acyl-binding pocket of aminoacylase-1." in: Biochemistry, Vol. 47, Issue 14, pp. 4266-75, 2008 (PubMed).

Figueiredo, Garcia Leão, De Oliveira et al.: "The amidase activity of human tissue kallikrein is significantly lower in the urine of patients with systolic heart failure." in: Journal of cardiac failure, Vol. 12, Issue 8, pp. 653-8, 2006 (PubMed).

Sass, Mohr, Olbrich et al.: "Mutations in ACY1, the gene encoding aminoacylase 1, cause a novel inborn error of metabolism." in: American journal of human genetics, Vol. 78, Issue 3, pp. 401-9, 2006 (PubMed).

Van Coster, Gerlo, Giardina et al.: "Aminoacylase I deficiency: a novel inborn error of metabolism." in: Biochemical and biophysical research communications, Vol. 338, Issue 3, pp. 1322-6, 2005 (PubMed).

Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network. ..." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).