|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Aminoacylase 1 (ACY1) antibody
|Synonyms||ACY1D, ACYLASE, Acy-1, 1110014J22Rik, Acy1, MGC55605, zgc:76955, ACY1, acy1, MGC86360, MGC127073, DKFZp469H2432|
Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||ACY1 (AAH00545, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: ACYLASE aminoacylase 1|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||ACY1 (AAH00545, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||45885 DA|
Background: Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cancer, Metabolism, Cell Structure|
|Restrictions||For Research Use only|
Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).
Van Coster, Gerlo, Giardina et al.: "Aminoacylase I deficiency: a novel inborn error of metabolism." in: Biochemical and biophysical research communications, Vol. 338, Issue 3, pp. 1322-6, 2005 (PubMed).
Sass, Mohr, Olbrich et al.: "Mutations in ACY1, the gene encoding aminoacylase 1, cause a novel inborn error of metabolism." in: American journal of human genetics, Vol. 78, Issue 3, pp. 401-9, 2006 (PubMed).
Figueiredo, Garcia Leão, De Oliveira et al.: "The amidase activity of human tissue kallikrein is significantly lower in the urine of patients with systolic heart failure." in: Journal of cardiac failure, Vol. 12, Issue 8, pp. 653-8, 2006 (PubMed).
Lindner, Alary, Wilke et al.: "Probing the acyl-binding pocket of aminoacylase-1." in: Biochemistry, Vol. 47, Issue 14, pp. 4266-75, 2008 (PubMed).
|Hosts||Rabbit (27), Mouse (11), Rat (2)|
|Reactivities||Human (36), Mouse (Murine) (25), Rat (Rattus) (23), Pig (Porcine) (20), Cow (Bovine) (18), Horse (Equine) (18), Dog (Canine) (4), Monkey (1)|
|Applications||Western Blotting (WB) (24), Immunofluorescence (IF) (21), ELISA (13), Flow Cytometry (FACS) (10), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1), Immunodiffusion (ID) (1), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Center (2), AA 1-408 (1), AA 2-408 (1)|