Aminoacylase 1 (ACY1) antibody

Antigen: Aminoacylase 1 (ACY1)

Synonyms: ACY1D, ACYLASE, Acy-1, 1110014J22Rik, Acy1, MGC55605, zgc:76955, ACY1, acy1, MGC86360, MGC127073, DKFZp469H2432

Clonality: Monoclonal (4F1-B7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396373

Additional Information

Alternative name: ACY1

Gene ID: 95

Immunogen: ACY1 (AAH00545, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 4F1-B7

Description: Other names: ACYLASE aminoacylase 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ACY1 (AAH00545, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 45885 DA

Comments:

Background: Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to ACY1 (AT1037a) on HeLa cell. [antibody concentration 10ug/ml] ACY1 monoclonal antibody (M01), clone 4F1-B7 Western Blot analysis of ACY1 expression in K-562 ( Cat # L009V1 ).

anti-Aminoacylase 1 (ACY1) antibody (Image 2)

Publications

Product: Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Van Coster, Gerlo, Giardina et al.: "Aminoacylase I deficiency: a novel inborn error of metabolism." in: Biochemical and biophysical research communications, Vol. 338, Issue 3, pp. 1322-6, 2005 (PubMed).

Sass, Mohr, Olbrich et al.: "Mutations in ACY1, the gene encoding aminoacylase 1, cause a novel inborn error of metabolism." in: American journal of human genetics, Vol. 78, Issue 3, pp. 401-9, 2006 (PubMed).

Figueiredo, Garcia Leão, De Oliveira et al.: "The amidase activity of human tissue kallikrein is significantly lower in the urine of patients with systolic heart failure." in: Journal of cardiac failure, Vol. 12, Issue 8, pp. 653-8, 2006 (PubMed).

Lindner, Alary, Wilke et al.: "Probing the acyl-binding pocket of aminoacylase-1." in: Biochemistry, Vol. 47, Issue 14, pp. 4266-75, 2008 (PubMed).