Histidine Ammonia-Lyase (HAL) antibody

Antigen: Histidine Ammonia-Lyase (HAL)

Synonyms: HIS, HSTD, hal, MGC79072, MGC89028, HAL, his, hstd, xhal

Clonality: Monoclonal (4F2)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396375

Additional Information

Alternative name: HAL

Gene ID: 3034

UniProt: NP_002099

Immunogen: HAL (NP_002099, 558 a.a. ~ 658 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4F2

Description: Other names: HIS, HSTD histidine ammonia-lyase

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: HAL (NP_002099, 558 a.a. ~ 658 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 72698 DA

Comments:

Background: Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of HAL expression in transfected 293T cell line by HAL monoclonal antibody (M04), clone 4F2. Lane 1: HAL transfected lysate(72.698 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Alemán, Ortíz, Langley et al.: "Regulation by glucagon of the rat histidase gene promoter in cultured rat hepatocytes and human hepatoblastoma cells." in: American journal of physiology. Endocrinology and metabolism, Vol. 289, Issue 1, pp. E172-9, 2005 (PubMed).

Kawai, Moriyama, Asai et al.: "Molecular characterization of histidinemia: identification of four missense mutations in the histidase gene." in: Human genetics, Vol. 116, Issue 5, pp. 340-6, 2005 (PubMed).

Eckhart, Schmidt, Mildner et al.: "Histidase expression in human epidermal keratinocytes: regulation by differentiation status and all-trans retinoic acid." in: Journal of dermatological science, Vol. 50, Issue 3, pp. 209-15, 2008 (PubMed).

Welsh, Karagas, Applebaum et al.: "A role for ultraviolet radiation immunosuppression in non-melanoma skin cancer as evidenced by gene-environment interactions." in: Carcinogenesis, Vol. 29, Issue 10, pp. 1950-4, 2008 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).