LIM and SH3 Protein 1 (LASP1) antibody

Antigen: LIM and SH3 Protein 1 (LASP1)

Synonyms: MLN50, Lasp-1, Def-4, SH3P6, AA408629, Tg(Col1a1-lacZ)1Ngma, fb92f05, zgc:77542, wu:fb92f05, LASP1, MGC128762, DKFZp459O1227, lasp1, MGC53336, MGC89667, mln50, lasp-1

Clonality: Monoclonal (4F5)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396389

Additional Information

Alternative name: LASP1

Gene ID: 3927

Immunogen: LASP1 (AAH12460, 1 a.a. ~ 262 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4F5

Description: Other names: Lasp-1, MLN50 LIM and SH3 protein 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: LASP1 (AAH12460, 1 a.a. ~ 262 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 29717 DA

Comments:

Background: This gene encodes a member of a LIM protein subfamily characterized by a LIM motif and a domain of Src homology region 3. The encoded protein functions as an actin-binding protein and possibly in cytoskeletal organization.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of LASP1 expression in transfected 293T cell line by LASP1 monoclonal antibody (M05), clone 4F5. Lane 1: LASP1 transfected lysate(29.7 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Wang, Feng, Xiao et al.: "LIM and SH3 protein 1 (Lasp1) is a novel p53 transcriptional target involved in hepatocellular carcinoma." in: Journal of hepatology, Vol. 50, Issue 3, pp. 528-37, 2009 (PubMed).

Salvi, Bongarzone, Miccichè et al.: "Proteomic identification of LASP-1 down-regulation after RNAi urokinase silencing in human hepatocellular carcinoma cells." in: Neoplasia (New York, N.Y.), Vol. 11, Issue 2, pp. 207-19, 2009 (PubMed).

Traenka, Hauck, Lewandrowski et al.: "Integrin-dependent translocation of LASP-1 to the cytoskeleton of activated platelets correlates with LASP-1 phosphorylation at tyrosine 171 by Src-kinase." in: Thrombosis and haemostasis, Vol. 102, Issue 3, pp. 520-8, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Frietsch, Grunewald, Jasper et al.: "Nuclear localisation of LASP-1 correlates with poor long-term survival in female breast cancer." in: British journal of cancer, Vol. 102, Issue 11, pp. 1645-53, 2010 (PubMed).