MAD2L1 Binding Protein (MAD2L1BP) antibody

Antigen: MAD2L1 Binding Protein (MAD2L1BP)

Synonyms: CMT2, KIAA0110, MGC11282, RP1-261G23.6, CMT2A, CMT2B, C76045, Mad2lbp, AU018945, AW550977, mKIAA0110, 0610009D16Rik, 2510031P20Rik, MGC109107, MAD2L1BP

Clonality: Monoclonal (4G11)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396419

Additional Information

Alternative name: MAD2L1BP

Gene ID: 9587

Immunogen: MAD2L1BP (AAH02904, 1 a.a. ~ 275 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4G11

Description: Other names: CMT2, KIAA0110, MGC11282, RP1-261G23.6 MAD2L1 binding protein

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MAD2L1BP (AAH02904, 1 a.a. ~ 275 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 31052 DA

Comments:

Background: The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

MAD2L1BP monoclonal antibody (M03), clone 4G11. Western Blot analysis of MAD2L1BP expression in Hela NE ( Cat # L013V3 ).

Publications

Product: Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Yun, Kim, Park et al.: "Mutation analysis of p31comet gene, a negative regulator of Mad2, in human hepatocellular carcinoma." in: Experimental & molecular medicine, Vol. 39, Issue 4, pp. 508-13, 2007 (PubMed).

Yang, Li, Tomchick et al.: "p31comet blocks Mad2 activation through structural mimicry." in: Cell, Vol. 131, Issue 4, pp. 744-55, 2007 (PubMed).

Venkatesan, Rual, Vazquez et al.: "An empirical framework for binary interactome mapping." in: Nature methods, Vol. 6, Issue 1, pp. 83-90, 2008 (PubMed).

Yun, Han, Yoon et al.: "p31comet Induces cellular senescence through p21 accumulation and Mad2 disruption." in: Molecular cancer research : MCR, Vol. 7, Issue 3, pp. 371-82, 2009 (PubMed).