MAD2L1 Binding Protein (MAD2L1BP) antibody

Details for Product No. ABIN396419
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Antigen
Synonyms CMT2, RP1-261G23.6, 0610009D16Rik, 2510031P20Rik, AU018945, AW550977, C76045, CMT2A, CMT2B, Mad2lbp, mKIAA0110, MAD2L1BP
Reactivity
Human
(22), (6), (3)
Host
Mouse
(19), (4)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(22), (8), (4), (4), (3)
Pubmed 5 references available
Catalog no. ABIN396419
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen MAD2L1BP (AAH02904, 1 a.a. ~ 275 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4G11
Isotype IgG2a kappa
Specificity MAD2L1BP (AAH02904, 1 a.a. ~ 275 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MAD2L1BP
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 31052 DA
Gene ID 12406
Research Area Cell Cycle, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-MAD2L1 Binding Protein (MAD2L1BP) antibody MAD2L1BP monoclonal antibody (M03), clone 4G11. Western Blot analysis of MAD2L1BP expression in Hela NE.
Background publications Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Yun, Kim, Park et al.: "Mutation analysis of p31comet gene, a negative regulator of Mad2, in human hepatocellular carcinoma." in: Experimental & molecular medicine, Vol. 39, Issue 4, pp. 508-13, 2007 (PubMed).

Yang, Li, Tomchick et al.: "p31comet blocks Mad2 activation through structural mimicry." in: Cell, Vol. 131, Issue 4, pp. 744-55, 2007 (PubMed).

Venkatesan, Rual, Vazquez et al.: "An empirical framework for binary interactome mapping...." in: Nature methods, Vol. 6, Issue 1, pp. 83-90, 2008 (PubMed).

Yun, Han, Yoon et al.: "p31comet Induces cellular senescence through p21 accumulation and Mad2 disruption." in: Molecular cancer research : MCR, Vol. 7, Issue 3, pp. 371-82, 2009 (PubMed).

Hosts (19), (4)
Reactivities (22), (6), (3)
Applications (22), (8), (4), (4), (3)
Epitopes (5), (3), (2), (2), (1)
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