Coagulation Factor III (thromboplastin, Tissue Factor) (F3) antibody

Antigen: Coagulation Factor III (thromboplastin, Tissue Factor) (F3)

Synonyms: TF, TFA, CD142, f3, Cf3, Cf-3, AA409063, F3, LOC396677, LOC100009127, tf

Clonality: Monoclonal (4G4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396435

Additional Information

Alternative name: F3

Gene ID: 2152

Immunogen: F3 (AAH11029, 45 a.a. ~ 155 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4G4

Description: Other names: CD142, TF, TFA coagulation factor III (thromboplastin, tissue factor)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: F3 (AAH11029, 45 a.a. ~ 155 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 33068 DA

Comments:

Background: This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of F3 expression in transfected 293T cell line by F3 monoclonal antibody (M01), clone 4G4. Lane 1: F3 transfected lysate(33.1 KDa). Lane 2: Non-transfected lysate. F3 monoclonal antibody (M01), clone 4G4 Western Blot analysis of F3 expression in A-431 ( Cat # L015V1 ).

anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) antibody (Image 2)

Publications

Product: Zwicker: "Predictive value of tissue factor bearing microparticles in cancer associated thrombosis." in: Thrombosis research, Vol. 125 Suppl 2, pp. S89-91, 2010 (PubMed).

Romero, Velez Edwards, Kusanovic et al.: "Identification of fetal and maternal single nucleotide polymorphisms in candidate genes that predispose to spontaneous preterm labor with intact membranes." in: American journal of obstetrics and gynecology, Vol. 202, Issue 5, pp. 431.e1-34, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Romero, Friel, Velez Edwards et al.: "A genetic association study of maternal and fetal candidate genes that predispose to preterm prelabor rupture of membranes (PROM)." in: American journal of obstetrics and gynecology, Vol. 203, Issue 4, pp. 361.e1-361.e30, 2010 (PubMed).

Bouchard, Mann, Butenas: "No evidence for tissue factor on platelets." in: Blood, Vol. 116, Issue 5, pp. 854-5, 2010 (PubMed).