Vesicle-Associated Membrane Protein 1 (Synaptobrevin 1) (VAMP1) antibody

Details for Product No. ABIN396511
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Antigen
Synonyms SYB1, VAMP-1, syb1, vamp-1, Syb1, lew, Syb-1, VAMP1, An12g07570
Reactivity
Human
(45), (29), (24), (17), (17), (14), (12), (12), (5), (1), (1)
Host
Mouse
(40), (20), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(48), (20), (16), (10), (10), (9), (4), (3)
Pubmed 5 references available
Catalog no. ABIN396511
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen VAMP1 (NP_055046, 28 a.a. ~ 97 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5A4
Isotype IgG2a lambda
Specificity VAMP1 (NP_055046, 28 a.a. ~ 97 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name VAMP1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 12902 DA
Gene ID 12406
UniProt NP_055046
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Vesicle-Associated Membrane Protein 1 (Synaptobrevin 1) (VAMP1) antibody Immunofluorescence of monoclonal antibody to VAMP1 on HepG2 cell (antibody concentration 10 µg/mL). VAMP1 monoclonal antibody (M02), clone 5A4 Western Blot analysis of VAMP1 expression in HepG2.
anti-Vesicle-Associated Membrane Protein 1 (Synaptobrevin 1) (VAMP1) antibody (2) anti-Vesicle-Associated Membrane Protein 1 (Synaptobrevin 1) (VAMP1) antibody (Image 2)
Background publications Pérez-Brangulí, Muhaisen, Blasi: "Munc 18a binding to syntaxin 1A and 1B isoforms defines its localization at the plasma membrane and blocks SNARE assembly in a three-hybrid system assay." in: Molecular and cellular neurosciences, Vol. 20, Issue 2, pp. 169-80, 2002 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Imabayashi, Mori, Gojo et al.: "Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis." in: Experimental cell research, Vol. 288, Issue 1, pp. 35-50, 2003 (PubMed).

Kiel, Demissie, Dupuis et al.: "Genome-wide association with bone mass and geometry in the Framingham Heart Study." in: BMC medical genetics, Vol. 8 Suppl 1, pp. S14, 2007 (PubMed).

Lu, Forsberg, Höög et al.: "Heterogeneous expression of SNARE proteins SNAP-23, SNAP-25, Syntaxin1 and VAMP in human parathyroid tissue." in: Molecular and cellular endocrinology, Vol. 287, Issue 1-2, pp. 72-80, 2008 (PubMed).

Hosts (40), (20), (3), (1)
Reactivities (45), (29), (24), (17), (17), (14), (12), (12), (5), (1), (1)
Applications (48), (20), (16), (10), (10), (9), (4), (3)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (2), (2), (2), (1), (1), (1), (1)
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