Baculoviral IAP Repeat-Containing 5 (BIRC5) (AA 1-101) antibody

Details for Product No. ABIN396525
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Antigen
Synonyms API4, EPR-1, AAC-11, Api4, TIAP, survivin40, AP14
Epitope
AA 1-101
(22), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(70), (27), (9), (1), (1), (1), (1)
Host
Mouse
(69), (17), (2)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(71), (30), (25), (15), (14), (12), (8), (6), (6), (5), (4), (3), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN396525
450.00 $
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Immunogen BIRC5 (NP_001159, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5B10
Isotype IgG2a kappa
Specificity BIRC5 (NP_001159, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BIRC5
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 16390 DA
Gene ID 12406
NCBI Accession NP_001159
Research Area Cancer, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.

Restrictions For Research Use only
Concentration 0.38mg/ml, 0.5mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Baculoviral IAP Repeat-Containing 5 (BIRC5) (AA 1-101) antibody Western Blot analysis of BIRC5 expression in transfected 293T cell line by BIRC5 monoclonal antibody (M01), clone 5B10. Lane 1: BIRC5 transfected lysate(16.4 KDa). Lane 2: Non-transfected lysate. Western blot analysis of BIRC5 over-expressed 293 cell line, cotransfected with BIRC5 Validated Chimera RNAi used as specificity and loading control.
anti-Baculoviral IAP Repeat-Containing 5 (BIRC5) (AA 1-101) antibody (2) anti-Baculoviral IAP Repeat-Containing 5 (BIRC5) (AA 1-101) antibody (Image 2)
Background publications Naumnik, Nilklińska, Ossolińska et al.: "Serum levels of HMGB1, survivin, and VEGF in patients with advanced non-small cell lung cancer during chemotherapy." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 47, Issue 4, pp. 703-9, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Yang, Sun, Cao et al.: "Survivin downregulation by siRNA/cationic liposome complex radiosensitises human hepatoma cells in vitro and in vivo." in: International journal of radiation biology, Vol. 86, Issue 6, pp. 445-57, 2010 (PubMed).

Yoshida, Zhang, Rivera Rosado et al.: "Blockade of Rac1 activity induces G1 cell cycle arrest or apoptosis in breast cancer cells through downregulation of cyclin D1, survivin, and X-linked inhibitor of apoptosis protein." in: Molecular cancer therapeutics, Vol. 9, Issue 6, pp. 1657-68, 2010 (PubMed).

Niu, Cheng, Ren et al.: "Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells to TRAIL-induced apoptosis." in: FEBS letters, Vol. 584, Issue 16, pp. 3519-24, 2010 (PubMed).

Validation Images
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