Baculoviral IAP Repeat-Containing 5 (BIRC5) antibody

Antigen: Baculoviral IAP Repeat-Containing 5 (BIRC5)

Synonyms: API4, EPR-1, AP14, BIRC5, MGC133855, DKFZp468N162, Api4, TIAP, AAC-11, survivin40, bir5a, survivin, bir1-A, svv1, XBIR1, Xsvv1, birc5.1-A, xSurvivin, XSurvivin1, LOC398389, LOC398418, Su2, birc5.1-B, BIR1, Survivin, Survivin1, xSurvivin1, SIX, Su1, api4, svv2, birc5, epr-1, xSurvivin2

Clonality: Monoclonal (5B10)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396525

Additional Information

Alternative name: BIRC5

Gene ID: 332

UniProt: NP_001159

Immunogen: BIRC5 (NP_001159, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 5B10

Description: Other names: API4, EPR1baculoviral IAP repeat-containing 5 (survivin)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BIRC5 (NP_001159, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 16390 DA

Comments:

Background: This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.

Synonyms: API4, EPR-1, AP14, BIRC5, MGC133855, DKFZp468N162, Api4, TIAP, AAC-11, survivin40, bir5a, survivin, bir1-A, svv1, XBIR1, Xsvv1, birc5.1-A, xSurvivin, XSurvivin1, LOC398389, LOC398418, Su2, birc5.1-B, BIR1, Survivin, Survivin1, xSurvivin1, SIX, Su1, api4, svv2, birc5, epr-1, xSurvivin2

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Concentration: 0.38mg/ml, 0.5mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of BIRC5 expression in transfected 293T cell line by BIRC5 monoclonal antibody (M01), clone 5B10. Lane 1: BIRC5 transfected lysate(16.4 KDa). Lane 2: Non-transfected lysate. Western blot analysis of BIRC5 over-expressed 293 cell line, cotransfected with BIRC5 Validated Chimera RNAi ( Cat # H00000332-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BIRC5 monoclonal antibody (M01), clone 5B10 (Cat # AT1299a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-Baculoviral IAP Repeat-Containing 5 (BIRC5) antibody (Image 2)

Publications

Product: Naumnik, Nilklińska, Ossolińska et al.: "Serum levels of HMGB1, survivin, and VEGF in patients with advanced non-small cell lung cancer during chemotherapy." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 47, Issue 4, pp. 703-9, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Yang, Sun, Cao et al.: "Survivin downregulation by siRNA/cationic liposome complex radiosensitises human hepatoma cells in vitro and in vivo." in: International journal of radiation biology, Vol. 86, Issue 6, pp. 445-57, 2010 (PubMed).

Yoshida, Zhang, Rivera Rosado et al.: "Blockade of Rac1 activity induces G1 cell cycle arrest or apoptosis in breast cancer cells through downregulation of cyclin D1, survivin, and X-linked inhibitor of apoptosis protein." in: Molecular cancer therapeutics, Vol. 9, Issue 6, pp. 1657-68, 2010 (PubMed).

Niu, Cheng, Ren et al.: "Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells to TRAIL-induced apoptosis." in: FEBS letters, Vol. 584, Issue 16, pp. 3519-24, 2010 (PubMed).