Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2) (AA 354-452) antibody

Details for Product No. ABIN396597
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Antigen
Synonyms
idh2, IDH2, F6P23.14, F6P23_14, IDH-II, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 2, isocitrate dehydrogenase II, isocitrate dehydrogenase subunit 2, D2HGA2, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-I ... show more
idh2, IDH2, F6P23.14, F6P23_14, IDH-II, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 2, isocitrate dehydrogenase II, isocitrate dehydrogenase subunit 2, D2HGA2, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH, wu:fb33c06, wu:fk31e05, wu:fq43b01, zgc:55485, E430004F23, IDPm, Idh-2 show less
Epitope
AA 354-452
(8), (8), (7), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(59), (32), (31), (13), (2), (2), (1), (1), (1), (1)
Host
Mouse
(43), (13), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(50), (34), (20), (14), (10), (3), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN396597
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Immunogen IDH2 (NP_002159, 354 a.a. ~ 452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5F11
Isotype IgG1 kappa
Specificity IDH2 (NP_002159, 354 a.a. ~ 452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name IDH2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 50909 DA
Gene ID 12406
NCBI Accession NP_002159
Research Area Metabolism, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2) (AA 354-452) antibody Immunoperoxidase of monoclonal antibody to IDH2 on formalin-fixed paraffin-embedded human colon (antibody concentration 3 µg/mL). Detection limit for recombinant GST tagged IDH2 is approximately 0.03 ng/mL as a capture antibody.
anti-Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2) (AA 354-452) antibody (2) anti-Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2) (AA 354-452) antibody (Image 2)
Background publications Paschka, Schlenk, Gaidzik et al.: "IDH1 and IDH2 mutations are frequent genetic alterations in acute myeloid leukemia and confer adverse prognosis in cytogenetically normal acute myeloid leukemia with NPM1 mutation without FLT3 internal tandem duplication." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 28, Issue 22, pp. 3636-43, 2010 (PubMed).

Lopez, Reitman, Solomon et al.: "IDH1(R132) mutation identified in one human melanoma metastasis, but not correlated with metastases to the brain." in: Biochemical and biophysical research communications, Vol. 398, Issue 3, pp. 585-7, 2010 (PubMed).

Boissel, Nibourel, Renneville et al.: "Prognostic impact of isocitrate dehydrogenase enzyme isoforms 1 and 2 mutations in acute myeloid leukemia: a study by the Acute Leukemia French Association group." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 28, Issue 23, pp. 3717-23, 2010 (PubMed).

Sellner, Capper, Meyer et al.: "Increased levels of 2-hydroxyglutarate in AML patients with IDH1-R132H and IDH2-R140Q mutations." in: European journal of haematology, Vol. 85, Issue 5, pp. 457-9, 2010 (PubMed).

Rocquain, Carbuccia, Trouplin et al.: "Combined mutations of ASXL1, CBL, FLT3, IDH1, IDH2, JAK2, KRAS, NPM1, NRAS, RUNX1, TET2 and WT1 genes in myelodysplastic syndromes and acute myeloid leukemias." in: BMC cancer, Vol. 10, pp. 401, 2010 (PubMed).

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