Glutaminyl-tRNA Synthetase (QARS) (AA 677-776) antibody

Details for Product No. ABIN396609
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Antigen
Synonyms Aast-g1n, BcDNA:GH11673, CG10506, Dmel\\CG10506, GlnRS, QRS, l(3)05461, qars, zgc:56395, EPRS, PSPTO3743, GLNRS, PRO2195, 1110018N24Rik, 1200016L19Rik, C80286, glnRS
Epitope
AA 677-776
(6), (4), (2), (1), (1), (1), (1)
Reactivity
Human
(21), (3), (3)
Host
Mouse
(12), (9)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(20), (12), (10), (5), (3), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN396609
450.00 $
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Immunogen QARS (NP_005042, 677 a.a. ~ 776 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5F5
Isotype IgG2b kappa
Specificity QARS (NP_005042, 677 a.a. ~ 776 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name QARS
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 87799 DA
Gene ID 12406
NCBI Accession NP_005042
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family.

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Glutaminyl-tRNA Synthetase (QARS) (AA 677-776) antibody QARS monoclonal antibody (M01), clone 5F5 Western Blot analysis of QARS expression in HeLa on formalin-fixed paraffin-embedded human colon (antibody concentration 3 µg/mL).
anti-Glutaminyl-tRNA Synthetase (QARS) (AA 677-776) antibody (2) anti-Glutaminyl-tRNA Synthetase (QARS) (AA 677-776) antibody (Image 2)
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Ling, Yao, Zheng et al.: "The C-terminal appended domain of human cytosolic leucyl-tRNA synthetase is indispensable in its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex." in: The Journal of biological chemistry, Vol. 280, Issue 41, pp. 34755-63, 2005 (PubMed).

Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome...." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Validation Images
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