Zinc Finger, BED-Type Containing 1 (ZBED1) antibody

Antigen: Zinc Finger, BED-Type Containing 1 (ZBED1)

Synonyms: ALTE, DREF, TRAMP, hDREF, KIAA0785, ZBED1, ZBED1P

Clonality: Monoclonal (5G1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396624

Additional Information

Alternative name: ZBED1

Gene ID: 9189

UniProt: NP_004720

Immunogen: ZBED1 (NP_004720, 3 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 5G1

Description: Other names: ALTE, KIAA0785, TRAMP zinc finger, BED-type containing 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ZBED1 (NP_004720, 3 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 78156 DA

Comments:

Background: This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to ZBED1 (AT4568a) on HeLa cell . [antibody concentration 10 ug/ml] Western Blot analysis of ZBED1 expression in transfected 293T cell line by ZBED1 monoclonal antibody (M01), clone 5G1. Lane 1: ZBED1 transfected lysate(78.2 KDa). Lane 2: Non-transfected lysate.

anti-Zinc Finger, BED-Type Containing 1 (ZBED1) antibody (Image 2)

Publications

Product: Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).

Yamashita, Komori, Higuchi et al.: "Human DNA replication-related element binding factor (hDREF) self-association via hATC domain is necessary for its nuclear accumulation and DNA binding." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7563-75, 2007 (PubMed).

Yamashita, Sano, Adachi et al.: "hDREF regulates cell proliferation and expression of ribosomal protein genes." in: Molecular and cellular biology, Vol. 27, Issue 6, pp. 2003-13, 2007 (PubMed).

Xin, Rual, Hirozane-Kishikawa et al.: "Shifted Transversal Design smart-pooling for high coverage interactome mapping." in: Genome research, Vol. 19, Issue 7, pp. 1262-9, 2009 (PubMed).