Zinc Finger, BED-Type Containing 1 (ZBED1) antibody

Details for Product No. ABIN396624
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(51), (12), (12), (12), (9), (2)
(39), (12)
Clonality (Clone)
Monoclonal ()
(3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Western Blotting (WB)
(40), (26), (10), (5), (5), (4), (3), (3), (2), (1)
Pubmed 5 references available
Catalog no. ABIN396624
Quantity 0.1 mg
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Immunogen ZBED1 (NP_004720, 3 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5G1
Isotype IgG2a kappa
Specificity ZBED1 (NP_004720, 3 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ZBED1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 78156 DA
Gene ID 12406
UniProt NP_004720
Research Area Cell Structure
Application Notes Western blot = 1:500-1000

Background: This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Zinc Finger, BED-Type Containing 1 (ZBED1) antibody Immunofluorescence of monoclonal antibody to ZBED1 on HeLa cell (antibody concentration 10 µg/mL). Western Blot analysis of ZBED1 expression in transfected 293T cell line by ZBED1 monoclonal antibody (M01), clone 5G1. Lane 1: ZBED1 transfected lysate(78.2 KDa). Lane 2: Non-transfected lysate.
anti-Zinc Finger, BED-Type Containing 1 (ZBED1) antibody (2) anti-Zinc Finger, BED-Type Containing 1 (ZBED1) antibody (Image 2)
Background publications Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).

Yamashita, Komori, Higuchi et al.: "Human DNA replication-related element binding factor (hDREF) self-association via hATC domain is necessary for its nuclear accumulation and DNA binding." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7563-75, 2007 (PubMed).

Yamashita, Sano, Adachi et al.: "hDREF regulates cell proliferation and expression of ribosomal protein genes." in: Molecular and cellular biology, Vol. 27, Issue 6, pp. 2003-13, 2007 (PubMed).

Xin, Rual, Hirozane-Kishikawa et al.: "Shifted Transversal Design smart-pooling for high coverage interactome mapping." in: Genome research, Vol. 19, Issue 7, pp. 1262-9, 2009 (PubMed).

Hosts (39), (12)
Reactivities (51), (12), (12), (12), (9), (2)
Applications (40), (26), (10), (5), (5), (4), (3), (3), (2), (1)
Conjugates (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (3), (2), (2), (2), (2)
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