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Zinc Finger, BED-Type Containing 1 (ZBED1) (AA 3-100) antibody

Details for Product No. ABIN396624, Supplier: Log in to see
Antigen
  • ALTE
  • DREF
  • hDREF
  • TRAMP
  • ZBED1
  • ZBED1P
Epitope
AA 3-100
8
5
3
3
2
2
2
1
1
1
Reactivity
Human
83
6
1
Host
Mouse
53
30
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
7
7
7
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
61
32
17
11
10
7
6
5
1
1
Supplier
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Immunogen ZBED1 (NP_004720, 3 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5G1
Isotype IgG2a kappa
Specificity ZBED1 (NP_004720, 3 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ZBED1 (ZBED1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 78156 DA
Gene ID 12406
NCBI Accession NP_004720
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Zinc Finger, BED-Type Containing 1 (ZBED1) (AA 3-100) antibody (ABIN396624) Immunofluorescence of monoclonal antibody to ZBED1 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-Zinc Finger, BED-Type Containing 1 (ZBED1) (AA 3-100) antibody (ABIN396624) anti-Zinc Finger, BED-Type Containing 1 (ZBED1) (AA 3-100) antibody (Image 2)
Background publications Xin, Rual, Hirozane-Kishikawa, Hill, Vidal, Boone, Thierry-Mieg: "Shifted Transversal Design smart-pooling for high coverage interactome mapping." in: Genome research, Vol. 19, Issue 7, pp. 1262-9, 2009

Yamashita, Sano, Adachi, Okamoto, Osada, Takahashi, Yamaguchi, Osumi, Hirose: "hDREF regulates cell proliferation and expression of ribosomal protein genes." in: Molecular and cellular biology, Vol. 27, Issue 6, pp. 2003-13, 2007

Yamashita, Komori, Higuchi, Yamaguchi, Osumi, Hirose: "Human DNA replication-related element binding factor (hDREF) self-association via hATC domain is necessary for its nuclear accumulation and DNA binding." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7563-75, 2007

Lim, Hao, Shaw, Patel, Szabó, Rual, Fisk, Li, Smolyar, Hill, Barabási, Vidal, Zoghbi: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006

Kimura, Wakamatsu, Suzuki, Ota, Nishikawa, Yamashita, Yamamoto, Sekine, Tsuritani, Wakaguri, Ishii, Sugiyama, Saito, Isono, Irie, Kushida, Yoneyama, Otsuka, Kanda, Yokoi, Kondo, Wagatsuma, Murakawa et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006