NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 5, 16kDa (NDUFB5) (AA 95-190) antibody

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Antigen
Synonyms hm:zeh0024, zgc:123331, 0610007D05Rik, AU015782, SGDH, CISGDH
Epitope
AA 95-190
(3), (2), (1), (1), (1)
Reactivity
Human
(9), (1), (1), (1), (1), (1), (1)
Host
Mouse
(6), (3)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(9), (3), (2), (2), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN396640
450.00 $
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Immunogen NDUFB5 (NP_002483, 95 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5G5
Isotype IgG1 kappa
Specificity NDUFB5 (NP_002483, 95 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name NDUFB5
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 21750 DA
Gene ID 12406
NCBI Accession NP_002483
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 5, 16kDa (NDUFB5) (AA 95-190) antibody Western Blot analysis of NDUFB5 expression in transfected 293T cell line by NDUFB5 monoclonal antibody (M01), clone 5G5. Lane 1: NDUFB5 transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.
Background publications Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Starr, Shiels, Harris et al.: "Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey." in: Mechanisms of ageing and development, Vol. 129, Issue 12, pp. 745-51, 2008 (PubMed).

Martins-de-Souza, Gattaz, Schmitt et al.: "Alterations in oligodendrocyte proteins, calcium homeostasis and new potential markers in schizophrenia anterior temporal lobe are revealed by shotgun proteome analysis." in: Journal of neural transmission (Vienna, Austria : 1996), Vol. 116, Issue 3, pp. 275-89, 2009 (PubMed).

Wang, McDonnell, Hebbring et al.: "Polymorphisms in mitochondrial genes and prostate cancer risk." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 17, Issue 12, pp. 3558-66, 2008 (PubMed).

Saito, Kawamoto, Kamatani: "Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects." in: Journal of human genetics, Vol. 54, Issue 6, pp. 317-23, 2009 (PubMed).

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