Variable Charge, X-Linked 3A (VCX3A) antibody

Antigen: Variable Charge, X-Linked 3A (VCX3A)

Synonyms: VCX3, VCXA, VCX-A, VCX8R, VCX-8r, MGC118976, MGC125730, MGC125796

Clonality: Monoclonal (6A3)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396659

Additional Information

Alternative name: VCX3A

Gene ID: 51481

UniProt: NP_057463

Immunogen: VCX3A (NP_057463, 2 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 6A3

Description: Other names: MGC118976, MGC125730, VCX8r, VCXA, VCX3, VCX8R, VCXA variable charge, X-linked 3A

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: VCX3A (NP_057463, 2 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 20020 DA

Comments:

Background: This gene belongs to the VCX/Y gene family, which has multiple members on both X and Y chromosomes, and all are expressed exclusively in male germ cells. The X-linked members are clustered on chromosome Xp22 and Y-linked members are two identical copies of the gene within a palindromic region on Yq11. The family members share a high degree of sequence identity, with the exception that a 30-bp unit is tandemly repeated in X-linked members but occurs only once in Y-linked members. The VCX gene cluster is polymorphic in terms of copy number, different individuals may have a different number of VCX genes. VCX/Y genes encode small and highly charged proteins of unknown function. The presence of a putative bipartite nuclear localization signal suggests that VCX/Y members are nuclear proteins. This gene contains 8 repeats of the 30-bp unit.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of VCX3A expression in transfected 293T cell line by VCX3A monoclonal antibody (M01), clone 6A3. Lane 1: VCX3A transfected lysate(17.8 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Wiemann, Arlt, Huber et al.: "From ORFeome to biology: a functional genomics pipeline." in: Genome research, Vol. 14, Issue 10B, pp. 2136-44, 2004 (PubMed).

Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Jiao, Wang, Kiledjian: "Identification of an mRNA-decapping regulator implicated in X-linked mental retardation." in: Molecular cell, Vol. 24, Issue 5, pp. 713-22, 2006 (PubMed).

Cuevas-Covarrubias, González-Huerta: "Analysis of the VCX3A, VCX2 and VCX3B genes shows that VCX3A gene deletion is not sufficient to result in mental retardation in X-linked ichthyosis." in: The British journal of dermatology, Vol. 158, Issue 3, pp. 483-6, 2008 (PubMed).

Jiao, Chen, Chen et al.: "Modulation of neuritogenesis by a protein implicated in X-linked mental retardation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 40, pp. 12419-27, 2009 (PubMed).