S100 Calcium Binding Protein A6 (S100A6) (Met10) antibody

Antigen: S100 Calcium Binding Protein A6 (S100A6)

Synonyms: 2A9, PRA, 5B10, CABP, CACY, S100A6

Binding Site: Met10

Clonality: Monoclonal (6B5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396682

Additional Information

Alternative name: S100A6

Gene ID: 6277

Immunogen: S100A6 (AAH01431, 1 a.a. ~ 91 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 6B5

Description: Other names: 2A9, 5B10, CABP, CACY, PRAS100 calcium binding protein A6 (calcyclin)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: S100A6 (AAH01431, 1 a.a. ~ 91 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 10180 DA

Comments:

Background: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to S100A6 (AT3759a) on HeLa cell . [antibody concentration 10 ug/ml] S100A6 monoclonal antibody (M10), clone 6B5 Western Blot analysis of S100A6 expression in HeLa ( Cat # L013V1 ).

anti-S100 Calcium Binding Protein A6 (S100A6) (Met10) antibody (Image 2)

Publications

Product: Ishii, Suzuki, Satomi et al.: "Increased cytoplasmic S100A6 expression is associated with pulmonary adenocarcinoma progression." in: Pathology international, Vol. 59, Issue 9, pp. 623-30, 2009 (PubMed).

Nedjadi, Kitteringham, Campbell et al.: "S100A6 binds to annexin 2 in pancreatic cancer cells and promotes pancreatic cancer cell motility." in: British journal of cancer, Vol. 101, Issue 7, pp. 1145-54, 2009 (PubMed).

Zimmermann, Biniossek, Maurer et al.: "Proteomic profiling in distinct cellular compartments of tumor cells reveals p53-dependent upregulation of S100A6 upon induction of telomere dysfunction." in: Proteomics, Vol. 9, Issue 22, pp. 5175-87, 2009 (PubMed).

Wei, Hoover, Ross et al.: "Serum S100A6 concentration predicts peritoneal tumor burden in mice with epithelial ovarian cancer and is associated with advanced stage in patients." in: PLoS ONE, Vol. 4, Issue 10, pp. e7670, 2009 (PubMed).

van Dieck, Lum, Teufel et al.: "S100 proteins interact with the N-terminal domain of MDM2." in: FEBS letters, Vol. 584, Issue 15, pp. 3269-74, 2010 (PubMed).