ERMAP antibody (erythroblast Membrane-Associated Protein (Scianna Blood Group)) (AA 376-476)

Details for Product anti-ERMAP Antibody No. ABIN396739, Supplier: Log in to see
Antigen
  • AA409279
  • AI666418
  • PRO2801
  • RD
  • SC
  • erythroblast membrane-associated protein
  • erythroblast membrane-associated protein (Scianna blood group)
  • LOC100354196
  • Ermap
  • ERMAP
Epitope
AA 376-476
13
11
10
9
8
8
4
3
2
2
2
1
1
Reactivity
Human
96
23
20
3
3
2
2
2
2
1
Host
Mouse
59
36
1
Clonality (Clone)
Monoclonal ()
Conjugate
This ERMAP antibody is un-conjugated
6
4
4
4
4
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Western Blotting (WB)
59
30
25
13
5
5
4
3
1
Options
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Immunogen ERMAP (NP_001017922, 376 a.a. ~ 476 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6F8
Isotype IgG2a kappa
Specificity ERMAP (NP_001017922, 376 a.a. ~ 476 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ERMAP (ERMAP Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 52605 DA
Gene ID 12406
NCBI Accession NP_001017922
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a cell surface transmembrane protein that may act as an erythroid cell receptor, possibly as a mediator of cell adhesion. Polymorphisms in this gene are responsible for the Scianna/Radin blood group system. Two transcript variants encoding the same protein have been found for this gene.

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-ERMAP antibody (erythroblast Membrane-Associated Protein (Scianna Blood Group)) (AA 376-476) (ABIN396739) ERMAP monoclonal antibody (M01), clone 6F8 Western Blot analysis of ERMAP expression ...
Immunohistochemistry (IHC) image for anti-ERMAP antibody (erythroblast Membrane-Associated Protein (Scianna Blood Group)) (AA 376-476) (ABIN396739) anti-erythroblast Membrane-Associated Protein (Scianna Blood Group) (ERMAP) (AA 376-476) antibody (Image 2)
Background publications Fuchisawa, Lomas-Francis, Hue-Roye, Reid: "The polymorphism nt 76 in exon 2 of SC is more frequent in whites than in blacks." in: Immunohematology / American Red Cross, Vol. 25, Issue 1, pp. 18-9, 2009 (PubMed).

Lin, He, Ye, He, Guan, Chen, Liang: "[Expression of human ermap gene in umbilical cord blood mononuclear cells during differentiation and development towards erythroid lineage]." in: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 16, Issue 2, pp. 328-32, 2008 (PubMed).

Gregory, Barlow, McLay, Kaul, Swarbreck, Dunham, Scott, Howe, Woodfine, Spencer, Jones, Gillson, Searle, Zhou, Kokocinski, McDonald, Evans, Phillips, Atkinson, Cooper, Jones, Hall, Andrews, Lloyd et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Flegel, Chen, Reid, Martin, Orsini, Poole, Moulds, Wagner: "SCER and SCAN: two novel high-prevalence antigens in the Scianna blood group system." in: Transfusion, Vol. 45, Issue 12, pp. 1940-4, 2005 (PubMed).

Yan, Zhu, Fu, He: "ABO, Rh, MNS, Duffy, Kidd,Yt, Scianna, and Colton blood group systems in indigenous Chinese." in: Immunohematology / American Red Cross, Vol. 21, Issue 1, pp. 10-4, 2005 (PubMed).