erythroblast Membrane-Associated Protein (Scianna Blood Group) (ERMAP) (AA 376-476) antibody

Details for Product No. ABIN396739
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Antigen
Synonyms AA409279, AI666418, PRO2801, RD, SC
Epitope
AA 376-476
(8), (7), (7), (4), (2), (2), (2), (2), (1), (1)
Reactivity
Human
(52), (20), (20), (2), (1), (1), (1), (1)
Host
Mouse
(39), (13)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(35), (19), (17), (10), (4), (3), (3), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN396739
450.00 $
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Immunogen ERMAP (NP_001017922, 376 a.a. ~ 476 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6F8
Isotype IgG2a kappa
Specificity ERMAP (NP_001017922, 376 a.a. ~ 476 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ERMAP
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 52605 DA
Gene ID 12406
NCBI Accession NP_001017922
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a cell surface transmembrane protein that may act as an erythroid cell receptor, possibly as a mediator of cell adhesion. Polymorphisms in this gene are responsible for the Scianna/Radin blood group system. Two transcript variants encoding the same protein have been found for this gene.

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-erythroblast Membrane-Associated Protein (Scianna Blood Group) (ERMAP) (AA 376-476) antibody ERMAP monoclonal antibody (M01), clone 6F8 Western Blot analysis of ERMAP expression in HeLa. Detection limit for recombinant GST tagged ERMAP is approximately 0.1 ng/mL as a capture antibody.
anti-erythroblast Membrane-Associated Protein (Scianna Blood Group) (ERMAP) (AA 376-476) antibody (2) anti-erythroblast Membrane-Associated Protein (Scianna Blood Group) (ERMAP) (AA 376-476) antibody (Image 2)
Background publications Yan, Zhu, Fu et al.: "ABO, Rh, MNS, Duffy, Kidd,Yt, Scianna, and Colton blood group systems in indigenous Chinese." in: Immunohematology / American Red Cross, Vol. 21, Issue 1, pp. 10-4, 2005 (PubMed).

Flegel, Chen, Reid et al.: "SCER and SCAN: two novel high-prevalence antigens in the Scianna blood group system." in: Transfusion, Vol. 45, Issue 12, pp. 1940-4, 2005 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Lin, He, Ye et al.: "[Expression of human ermap gene in umbilical cord blood mononuclear cells during differentiation and development towards erythroid lineage]." in: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 16, Issue 2, pp. 328-32, 2008 (PubMed).

Fuchisawa, Lomas-Francis, Hue-Roye et al.: "The polymorphism nt 76 in exon 2 of SC is more frequent in whites than in blacks." in: Immunohematology / American Red Cross, Vol. 25, Issue 1, pp. 18-9, 2009 (PubMed).

Validation Images
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