Acid Phosphatase 2, Lysosomal (ACP2) antibody

Antigen: Acid Phosphatase 2, Lysosomal (ACP2)

Synonyms: LAP, Acp-2, ACP2, MGC142461, MGC145702, DKFZp459L1010

Clonality: Monoclonal (M1-4A12)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396836

Additional Information

Alternative name: ACP2

Gene ID: 53

Immunogen: ACP2 (AAH03160, 1 a.a. ~ 424 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b lambda

Clone: M1-4A12

Description: Other names: acid phosphatase 2, lysosomal

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ACP2 (AAH03160, 1 a.a. ~ 424 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 48344 DA

Comments:

Background: This gene encodes the beta subunit of lysosomal acid phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Mutations in this gene or in the related alpha subunit gene cause acid phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to ACP2 (AT1028a) on HeLa cell. [antibody concentration 10ug/ml] Western Blot analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12. Lane 1: ACP2 transfected lysate(48.3 KDa). Lane 2: Non-transfected lysate.

anti-Acid Phosphatase 2, Lysosomal (ACP2) antibody (Image 2)

Publications

Product: Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Melquist, Craig, Huentelman et al.: "Identification of a novel risk locus for progressive supranuclear palsy by a pooled genomewide scan of 500,288 single-nucleotide polymorphisms." in: American journal of human genetics, Vol. 80, Issue 4, pp. 769-78, 2007 (PubMed).

Wang, McDonnell, Hebbring et al.: "Polymorphisms in mitochondrial genes and prostate cancer risk." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 17, Issue 12, pp. 3558-66, 2008 (PubMed).