BANF1 antibody (Barrier To Autointegration Factor 1) (AA 1-90)

Details for Product anti-BANF1 Antibody No. ABIN396842, Supplier: Log in to see
Antigen
  • BAF
  • CG7380
  • Dmel\\CG7380
  • anon-WO0172774.178
  • dBAF
  • BANF1
  • DKFZp468C097
  • baf
  • bcrp1
  • BCRP1
  • D14S1460
  • NGPS
  • Baf
  • Bcrp1
  • C78287
  • L2bp1/Baf
  • L2bp1
  • fa01c01
  • wu:fa01c01
  • zgc:77767
  • barrier to autointegration factor
  • barrier to autointegration factor 1
  • baf
  • BANF1
  • banf1
  • Banf1
Epitope
AA 1-90
15
10
7
7
7
6
5
4
2
1
1
Reactivity
Human
75
36
27
2
2
2
2
2
2
1
1
1
1
Host
Mouse
63
12
Clonality (Clone)
Monoclonal ()
Conjugate
This BANF1 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
62
37
23
20
18
13
4
3
2
1
Options
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Immunogen BANF1 (AAH05942, 1 a.a. ~ 90 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone M2
Isotype IgG2a kappa
Specificity BANF1 (AAH05942, 1 a.a. ~ 90 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BANF1 (BANF1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 10059 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-BANF1 antibody (Barrier To Autointegration Factor 1) (AA 1-90) (ABIN396842) Immunofluorescence of monoclonal antibody to BANF1 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-BANF1 antibody (Barrier To Autointegration Factor 1) (AA 1-90) (ABIN396842) anti-Barrier To Autointegration Factor 1 (BANF1) (AA 1-90) antibody (Image 2)
Background publications Montes de Oca, Shoemaker, Gucek, Cole, Wilson: "Barrier-to-autointegration factor proteome reveals chromatin-regulatory partners." in: PLoS ONE, Vol. 4, Issue 9, pp. e7050, 2009 (PubMed).

Haraguchi, Kojidani, Koujin, Shimi, Osakada, Mori, Yamamoto, Hiraoka: "Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly." in: Journal of cell science, Vol. 121, Issue Pt 15, pp. 2540-54, 2008 (PubMed).

Wiebe, Traktman: "Poxviral B1 kinase overcomes barrier to autointegration factor, a host defense against virus replication." in: Cell host & microbe, Vol. 1, Issue 3, pp. 187-97, 2007 (PubMed).

Haraguchi, Koujin, Osakada, Kojidani, Mori, Masuda, Hiraoka: "Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells." in: Journal of cell science, Vol. 120, Issue Pt 12, pp. 1967-77, 2007 (PubMed).

Li, Craigie: "Virology: HIV goes nuclear." in: Nature, Vol. 441, Issue 7093, pp. 581-2, 2006 (PubMed).