Barrier To Autointegration Factor 1 (BANF1) (AA 1-90) antibody

Details for Product No. ABIN396842
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Antigen
Synonyms BAF, CG7380, Dmel\\CG7380, anon-WO0172774.178, dBAF, BANF1, DKFZp468C097, baf, bcrp1, BCRP1, D14S1460, NGPS, Baf, Bcrp1, C78287, L2bp1/Baf, L2bp1, fa01c01, wu:fa01c01, zgc:77767
Epitope
AA 1-90
(13), (8), (7), (7), (5), (4), (1), (1)
Reactivity
Human
(60), (33), (22), (12), (12)
Host
Mouse
(49), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(49), (34), (23), (16), (10), (6), (3), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN396842
450.00 $
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Immunogen BANF1 (AAH05942, 1 a.a. ~ 90 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone M2
Isotype IgG2a kappa
Specificity BANF1 (AAH05942, 1 a.a. ~ 90 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BANF1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 10059 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Barrier To Autointegration Factor 1 (BANF1) (AA 1-90) antibody Immunofluorescence of monoclonal antibody to BANF1 on HeLa cell (antibody concentration 10 µg/mL). BANF1 monoclonal antibody (M07), clone M2 Western Blot analysis of BANF1 expression in Jurkat.
anti-Barrier To Autointegration Factor 1 (BANF1) (AA 1-90) antibody (2) anti-Barrier To Autointegration Factor 1 (BANF1) (AA 1-90) antibody (Image 2)
Background publications Li, Craigie: "Virology: HIV goes nuclear." in: Nature, Vol. 441, Issue 7093, pp. 581-2, 2006 (PubMed).

Haraguchi, Koujin, Osakada et al.: "Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells." in: Journal of cell science, Vol. 120, Issue Pt 12, pp. 1967-77, 2007 (PubMed).

Wiebe, Traktman: "Poxviral B1 kinase overcomes barrier to autointegration factor, a host defense against virus replication." in: Cell host & microbe, Vol. 1, Issue 3, pp. 187-97, 2007 (PubMed).

Haraguchi, Kojidani, Koujin et al.: "Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly." in: Journal of cell science, Vol. 121, Issue Pt 15, pp. 2540-54, 2008 (PubMed).

Montes de Oca, Shoemaker, Gucek et al.: "Barrier-to-autointegration factor proteome reveals chromatin-regulatory partners." in: PLoS ONE, Vol. 4, Issue 9, pp. e7050, 2009 (PubMed).

Validation Images
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