Barrier To Autointegration Factor 1 (BANF1) antibody

Antigen: Barrier To Autointegration Factor 1 (BANF1)

Synonyms: BAF, BCRP1, D14S1460, MGC111161, Baf, Bcrp1, L2bp1, L2bp1/Baf, MGC105365, BANF1, DKFZp468C097, C78287, baf, banf1, fa01c01, wu:fa01c01

Clonality: Monoclonal (M2)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396842

Additional Information

Alternative name: BANF1

Gene ID: 8815

Immunogen: BANF1 (AAH05942, 1 a.a. ~ 90 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: M2

Description: Other names: BAF, BCRP1, D14S1460 barrier to autointegration factor 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BANF1 (AAH05942, 1 a.a. ~ 90 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 10059 DA

Comments:

Background: The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to BANF1 ( Cat# AT1267a ) on HeLa cell . [antibody concentration 10 ug/ml] BANF1 monoclonal antibody (M07), clone M2 Western Blot analysis of BANF1 expression in Jurkat ( Cat # L017V1 ).

anti-Barrier To Autointegration Factor 1 (BANF1) antibody (Image 2)

Publications

Product: Li, Craigie: "Virology: HIV goes nuclear." in: Nature, Vol. 441, Issue 7093, pp. 581-2, 2006 (PubMed).

Haraguchi, Koujin, Osakada et al.: "Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells." in: Journal of cell science, Vol. 120, Issue Pt 12, pp. 1967-77, 2007 (PubMed).

Wiebe, Traktman: "Poxviral B1 kinase overcomes barrier to autointegration factor, a host defense against virus replication." in: Cell host & microbe, Vol. 1, Issue 3, pp. 187-97, 2007 (PubMed).

Haraguchi, Kojidani, Koujin et al.: "Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly." in: Journal of cell science, Vol. 121, Issue Pt 15, pp. 2540-54, 2008 (PubMed).

Montes de Oca, Shoemaker, Gucek et al.: "Barrier-to-autointegration factor proteome reveals chromatin-regulatory partners." in: PLoS ONE, Vol. 4, Issue 9, pp. e7050, 2009 (PubMed).