Fatty Acid Synthase (FASN) antibody

Antigen: Fatty Acid Synthase (FASN)

Synonyms: FASN, FAS, fj34h12, wu:fj34h12, Cgl0836, A630082H08Rik, OA-519, SDR27X1, MGC14367, MGC15706, BcDNA:GH07626, DM_7295848, Fatty acid synthase, anon-EST:Posey235, anon-EST:Posey43, anon-WO0138581.1, DmelCG3523

Clonality: Monoclonal (S1)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396850

Additional Information

Alternative name: FASN

Gene ID: 2194

Immunogen: FASN (AAH07909, 1 a.a. ~ 440 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: S1

Description: Other names: FAS, MGC14367, MGC15706, OA-519 fatty acid synthase

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: FASN (AAH07909, 1 a.a. ~ 440 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 273400 DA

Comments:

Background: The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Synonyms: FASN, FAS, fj34h12, wu:fj34h12, Cgl0836, A630082H08Rik, OA-519, SDR27X1, MGC14367, MGC15706, BcDNA:GH07626, DM_7295848, Fatty acid synthase, anon-EST:Posey235, anon-EST:Posey43, anon-WO0138581.1, DmelCG3523

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody (M03), clone S1. Lane 1: FASN transfected lysate(48.3 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Fernandez-Real, Menendez, Moreno-Navarrete et al.: "Extracellular fatty acid synthase: a possible surrogate biomarker of insulin resistance." in: Diabetes, Vol. 59, Issue 6, pp. 1506-11, 2010 (PubMed).

Zeng, Biernacka, Holly et al.: "Hyperglycaemia confers resistance to chemotherapy on breast cancer cells: the role of fatty acid synthase." in: Endocrine-related cancer, Vol. 17, Issue 2, pp. 539-51, 2010 (PubMed).

Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Nguyen, Ma, Chavarro et al.: "Fatty acid synthase polymorphisms, tumor expression, body mass index, prostate cancer risk, and survival." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 28, Issue 25, pp. 3958-64, 2010 (PubMed).