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Fatty Acid Synthase (FASN) antibody
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||FASN (AAH07909, 1 a.a. ~ 440 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: FAS, MGC14367, MGC15706, OA-519 fatty acid synthase|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||FASN (AAH07909, 1 a.a. ~ 440 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||273400 DA|
Background: The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
|Synonyms||FASN, FAS, fj34h12, wu:fj34h12, Cgl0836, A630082H08Rik, OA-519, SDR27X1, MGC14367, MGC15706, BcDNA:GH07626, DM_7295848, Fatty acid synthase, anon-EST:Posey235, anon-EST:Posey43, anon-WO0138581.1, DmelCG3523|
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cancer, Cell Structure|
|Restrictions||For Research Use only|
|Western Blot analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody (M03), clone S1. Lane 1: FASN transfected lysate(48.3 KDa). Lane 2: Non-transfected lysate.|
Fernandez-Real, Menendez, Moreno-Navarrete et al.: "Extracellular fatty acid synthase: a possible surrogate biomarker of insulin resistance." in: Diabetes, Vol. 59, Issue 6, pp. 1506-11, 2010 (PubMed).
Zeng, Biernacka, Holly et al.: "Hyperglycaemia confers resistance to chemotherapy on breast cancer cells: the role of fatty acid synthase." in: Endocrine-related cancer, Vol. 17, Issue 2, pp. 539-51, 2010 (PubMed).
Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).
Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).
Nguyen, Ma, Chavarro et al.: "Fatty acid synthase polymorphisms, tumor expression, body mass index, prostate cancer risk, and survival." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 28, Issue 25, pp. 3958-64, 2010 (PubMed).
|Hosts||Rabbit (18), Goat (3), Mouse (3)|
|Reactivities||Human (22), Mouse (Murine) (11), Rat (Rattus) (7), Chicken (2), Cat (Feline) (1), Cow (Bovine) (1), Dog (Canine) (1), Pig (Porcine) (1)|
|Applications||Western Blotting (WB) (22), ELISA (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (IHC) (5), Immunofluorescence (IF) (4), Flow Cytometry (FACS) (3), Immunocytochemistry (ICC) (3), Immunoprecipitation (IP) (3)|
|Epitopes||C-Term (2), Internal Region (2), N-Term (2), AA 10-212 (1), Center (1)|