Actin, beta (ACTB) antibody (HRP)

Antigen: Actin, beta (ACTB)

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Clonality: Monoclonal (2D1D10)

Host: Mouse

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Certificates: ISO 9001:2008

Catalog no.: ABIN396861

Additional Information

Alternative name: beta actin

Immunogen: A synthetic peptide DDDIAALVVDNGSG coupled to KLH

Format: Purified

Isotype: IgG2a

Clone: 2D1D10

Description: Antibodies-Online Anti-beta-Actin [HRP] Monoclonal Antibody (Mouse) is HRP conjugated Anti-beta-Actin Monoclonal Antibody (Mouse) (ABIN396859) purified from mice ascites by protein G affinity column. This product provides a useful and serves as a specific tool in the study of the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Do not add azide because it inhibits the activity of HRP.

Specificity: Anti-beta-Actin [HRP] Monoclonal Antibody (Mouse) reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig substrates. It has not yet been tested in other species.

Synonyms: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.1-1.0 ug/ml Western blot: 0.1-1.0 ug/ml Other applications: user-optimized

Concentration: 40 ug, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal

Purification: Protein G affinity column

Storage: Anti-beta-Actin [HRP] Monoclonal Antibody (Mouse) should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Restrictions: For Research Use only

Images

Multiple Tag Cell Lysate (Antibodies-Online, M0100)Primary antibody:A. 1 µg/ml Anti-c-Myc-tag Monoclonal Antibody (Mouse) (Antibodies-Online, ABIN396860)B. 1 µg/ml Anti-c-Myc-tag Monoclonal Antibody (Mouse) (From competitor A)Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (Antibodies-Online, ABIN398387, 1:10,000)

Loading:1. Lysate that plasmid containing GST-c-Myc transfected in 293 cells2. Lysate that plasmid containing c-Myc-GST transfected in 293 cellsPrimary antibody:A. 1 µg/ml Mouse Anti-c-Myc-tag Monoclonal Antibody (Antibodies-Online, ABIN396860)B. 1 µg/ml Mouse Anti c-Myc Monoclonal Antibody (From competitor A)Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (Antibodies-Online, ABIN398387, 1:10,000)

Lane 1-2: Multiplex tag cell lysate (Antibodies-Online, M0100)NC: 293 cell lysatePrimary antibody: Anti-c-Myc-tag Monoclonal Antibody (Mouse) (Antibodies-Online, ABIN396860)The Western was performed using One-Step Western Blot Kit (Antibodies-Online, L00205) with 0.9 µg of the antibody added to diluted WB solution (dilute 1.5 ml of WB with 1.5 ml of PBST)