Green Fluorescent Protein (GFP) antibody

Details for Product No. ABIN398304
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Antigen
Reactivity
Aequorea victoria
(341), (31), (11), (4), (3), (3), (2), (1), (1)
Host
Rat
(148), (147), (56), (26), (4), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(24), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA
(300), (190), (107), (62), (59), (47), (38), (29), (29), (16), (13), (10), (8), (4), (3), (2)
Pubmed 6 references available
Catalog no. ABIN398304
Quantity 100 µL
Price
244.20 $   Plus shipping costs $45.00
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Purpose For biochemical analysis of GFP-tagged fusion proteins.
Clone 3H9
Isotype IgG2a
Specificity 3H9 efficiently recognizes green fluorescent proteins from Aequorea victoria such as eGFP, wtGFP, YFP, or CFP, but no red fluorescent proteins.
Characteristics Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells in particular the prominent green fluorescent protein (GFP) from Aequorea victoria.
Here we offer a rat monoclonal antibody (MAb) against various GFPs, designated as 3H9. The 3H9 MAb is characterized by high affinity and specificity. It is suitable for immunoblotting and ELISA.
With this versatile antibody GFP and fusions thereof become attractive tools for biochemical applications like identification of interacting proteins or Western Blotting.
Purification Hybridoma supernatant. The Hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kDa exclusion membrane to an antibody concentration of 1 mg/ml.
Background The first green fluorescent protein (GFP) was isolated from Aequorea victoria and has a molecular weight of 27 kDa. Genetic mutations of GFP resulted in fluorescent proteins with different excitation and emission spectra e.g. eGFP, YFP, CFP. Fluorescent proteins became attractive tools for a variety of applications.
Research Area Tags/Labels
Application Notes Western Blot: Used at a dilution of 1/1000. Detects a band of approximately 31 kDa (the predicted molecular weight of GFP).
Comment

IF: not working

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Preservative Sodium azide
Handling Advice Do not freeze.
Storage 4 °C
Expiry Date 12 months
Validation Images
anti-Green Fluorescent Protein (GFP) antibody anti-Green Fluorescent Protein (GFP) antibody
Supplier Images
anti-Green Fluorescent Protein (GFP) antibody Western Blot analysis using anti-GFP antibody 3H9. Primary antibody: 0.2 ug of GFP antibody 3H9 incubation for 1 h at RT. Secondary antibody: anti-rat-HRP (Jackson ImmunoResearch Europe). Detection with ECL Western Blot Detection Kit
Product cited in: Sieber, Lange, Kollmorgen et al.: "Sharpin Contributes to TNFα Dependent NFκB Activation and Anti-Apoptotic Signalling in Hepatocytes." in: PLoS ONE, Vol. 7, Issue 1, pp. e29993, 2012 (PubMed).

Garcia-Santisteban, Zorroza, Rodriguez: "Two nuclear localization signals in USP1 mediate nuclear import of the USP1/UAF1 complex." in: PLoS ONE, Vol. 7, Issue 6, pp. e38570, 2012 (PubMed).

Bouasker, Simard: "The slicing activity of miRNA-specific Argonautes is essential for the miRNA pathway in C. elegans." in: Nucleic acids research, 2012 (PubMed).

Becker, Allmann, Hofstätter et al.: "Direct homo- and hetero-interactions of MeCP2 and MBD2." in: PLoS ONE, Vol. 8, Issue 1, pp. e53730, 2013 (PubMed).

Lestini, Laptenok, Kühn et al.: "Intracellular dynamics of archaeal FANCM homologue Hef in response to halted DNA replication." in: Nucleic acids research, 2013 (PubMed).

Kríz, Pospíchalová, Masek et al.: "β-Arrestin Promotes Wnt-induced Low Density Lipoprotein Receptor-related Protein 6 (Lrp6) Phosphorylation via Increased Membrane Recruitment of Amer1 Protein." in: The Journal of biological chemistry, Vol. 289, Issue 2, pp. 1128-41, 2014 (PubMed).

Hosts (148), (147), (56), (26), (4), (2)
Reactivities (341), (31), (11), (4), (3), (3), (2), (1), (1)
Applications (300), (190), (107), (62), (59), (47), (38), (29), (29), (16), (13), (10), (8), (4), (3), (2)
Conjugates (24), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (26), (18), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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