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Green Fluorescent Protein (GFP) antibody

Antigen

Green Fluorescent Protein (GFP)
Clonality Monoclonal (3H9)
Host
Application
Alternatives Western Blotting (WB), ELISA
1 reference available
Catalog no. ABIN398304
Quantity 100ug  (1mg/ml)
Price 273.00 $   Plus shipping costs $35.00
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anti-Green Fluorescent Protein (GFP) antibody

Additional Information
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Characteristics Rat monoclonal [3H9] to Green Fluorescent Proteins. For biochemical and cell biological analysis of GFP-tagged fusion proteins.
Format Supernatant
Isotype IgG2a  (Matching secondary antibodies)
Clone 3H9
Description Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria. Here we offer a rat monoclonal antibody (MAb) against green FPs, designated as 3H9. The 3H9 MAb is characterized by high affinity and specificity against GFP. It is suitable for immunoblotting and ELISA. With this versatile antibody GFP and fusions thereof become attractive tools for biochemical applications like identification of interacting proteins or Western Blotting.

Application Details
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Application Notes Western Blot: Used at a dilution of 1/1000. Detects a band of approximately 31 kDa (the predicted molecular weight of GFP).
Concentration 1mg/ml
Purity Liquid. hybridoma supernatant. The hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kD exclusion membrane to an antibody concentration of 1 mg/ml.
Buffer Preservative: 0.01% Sodium Azide
Storage Shipped at RT. Upon delivery aliquot and store at -20°C. Store at 4°C short term (4-8 weeks). Avoid freeze / thaw cycles.
Research Area Tags/Labels
Restrictions For Research Use only

Publications
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Publications Sieber, Lange, Kollmorgen et al.: "Sharpin Contributes to TNFα Dependent NFκB Activation and Anti-Apoptotic Signalling in Hepatocytes." in: PLoS ONE, Vol. 7, Issue 1, pp. e29993, 2012 (PubMed).

Alternatives
Application Details Publications Images Secondary Antibodies back to top

Alternatives for antigen "Green Fluorescent Protein (GFP)", type "Antibodies"
Hosts Mouse (12), Rabbit (10), Goat (9), Chicken (2), Sheep (2)
Reactivities Tag (2), Jellyfish (1)
Applications Western Blotting (WB) (32), ELISA (27), Immunoprecipitation (IP) (15), Immunohistochemistry (IHC) (7), Immunofluorescence (IF) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
Conjugates Biotin (3), FITC (3), Alkaline Phosphatase (AP) (2), HRP (2), Peroxidase (POD) (2), Texas Red (TR) (2), TRITC (1)