NF H antibody

Antigen: NF H

Clonality: Polyclonal

Host: Rabbit

Reactivity: Various Species

Application: Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN398362

Additional Information

Alternative name: NF-H

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200,000-220,000 Da, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyothrophic lateral sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease.

Specificity: Phosphorylated NF-H from a variety of species

Application Details

Application Notes: Immunofluorescence microscopy: 1:500Western blot: 1:1,000 The antibody has also been used successfully in the detection role in ELISA.

Concentration: 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide. Frozen liquid

Purification: Immunoaffinity purification

Storage: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.

Restrictions: For Research Use only