Internexin Neuronal Intermediate Filament Protein, alpha (INA) antibody

Antigen: Internexin Neuronal Intermediate Filament Protein, alpha (INA)

Synonyms: NEF5, NF-66, TXBP-1, FLJ18662, FLJ57501, MGC12702, Nf66, Inexa, Intlaa, NF66, AV028420, MGC25352, Ina, cb126, sb:cb126, ina-A, xefiltin, ina-b, MGC53005, ina-a, inexa, MGC82496, ina, XNIF, CTDSPL, MGC69277, INA, MGC142838, LOC555251, nef5, xina, nf-66, txbp-1

Clonality: Polyclonal

Host: Chicken

Reactivity: Human, Rat (Rattus), Mouse (Murine), Cat (Feline), Mammalian

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN398377

Additional Information

Alternative name: Internexin/NF66

Format: Affinity-purified

Isotype: TBD  (Matching secondary antibodies)

Description: Neurofilaments can be defined as the intermediate or 10 nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, a-internexin, nestin and in some cases vimentin. A-internexin is a ~66,000 kDa Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of a-internexin during neuronal regeneration. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. The antiserum was raised against recombinant alpha-internexin expressed in and purified from E. coli.

Specificity: Alpha-internexin (a.k.a. NF66) in human, rat, mouse, cat and other mammals

Synonyms: NEF5, NF-66, TXBP-1, FLJ18662, FLJ57501, MGC12702, Nf66, Inexa, Intlaa, NF66, AV028420, MGC25352, Ina, cb126, sb:cb126, ina-A, xefiltin, ina-b, MGC53005, ina-a, inexa, MGC82496, ina, XNIF, CTDSPL, MGC69277, INA, MGC142838, LOC555251, nef5, xina, nf-66, txbp-1

Application Details

Application Notes: WB: 1:5,000 IF: 1:500

Concentration: 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid

Purification: Immunoaffinity purification

Storage: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.

Restrictions: For Research Use only