Fibrillarin (FBL) antibody

Antigen: Fibrillarin (FBL)

Synonyms: zgc:56145, zgc:77130, wu:fb37g12, MGC116371, FBL, FIB, FLRN, RNU3IP1, AL022665, CG9888, DmelCG9888, Fibri, GCR-6, GCR6, pen59C5, Pen59C5, MGC76139

Clonality: Monoclonal (A00153-01)

Host: Mouse

Reactivity: Human, Rat (Rattus), Plant, Fruit Fly (Drosophila melanogaster), Caenorhabditis elegans (C. elegans), Schizosaccharomyces pombe (S. pombe)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN398380

Additional Information

Alternative name: Fibrillarin

Format: Affinity-purified

Clone: A00153-01

Description: Nop1p/Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of two extended beta-sheets flanked by four alpha-helices. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34,000 Da protein, which has been subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Specificity: This antibody cross-reacts with fibrillarin in human, rat, plant, drosophila, C. elegans and S. pombe.

Application Details

Application Notes: Western blot of yeast protein samples: 1:1,000 (cell lysates) to 1:5,000 (nuclear fractions), followed by chemiluminescent detection (ECL)Other (non-ECL) Western detection methods: 1:500 to 1:2,500Detection mammalian fibrillarin on Western blot by ECL: 1:250Immunofluorescence on yeast cells: 1:500 to 1:2,500Immunofluorescence of mammalian cells: 1:250

Concentration: 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid

Storage: This product will remain stable for one year from the date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquots will remain stable for three months if stored at 4°C.

Research Area: Chromatin and Nuclear Signaling, Chromatin Binding Proteins

Restrictions: For Research Use only

Images

Rat neurons and glia stained with Mouse Anti-Fibrillarin Monoclonal Antibody (Antibodies-Online, ABIN398380) (green) and chicken NF-H antibody (red).Nuclear DNA is revealed with Hoechst dye (blue).